Recombinant Human BCMA/TNFRSF17 Protein, Atto 488 Conjugate

Catalog #: ATJ193 Datasheet / COA / SDS

Fc Chimera

Catalog #	Availability	Size / Price	Qty
ATJ193-020

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BCMA/TNFRSF17 Protein, Atto 488 Conjugate Summary

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Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by flow cytometry for its ability to bind anti-Human BCMA Monoclonal Antibody conjugated fluorescent beads.

Source

Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein

Human BCMA/TNFRSF17 (Met1-Ala54) Accession # Q6PE46	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q6PE46

N-terminal Sequence
Analysis

Met1

Structure / Form

Disulfide-linked homodimer, labeled with Atto 488 via amines

Excitation Wavelength: 501 nm

Emission Wavelength: 523 nm

Predicted Molecular Mass

32 kDa (monomer)

SDS-PAGE

35-45 kDa, under reducing conditions

Product Datasheets

ATJ193

Product Datasheet

COA

SDS

ATJ193

Formulation	Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Flow Cytometry

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Fluorescent beads conjugated to anti-Human BCMA Monoclonal Antibody were stained with (A) Recombinant Human BCMA/TNFRSF17 Fc Chimera Atto 488 Protein (Catalog # ATJ193) or (B) unstained.

SDS-PAGE

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2 μg/lane of Recombinant Human BCMA/TNFRSF17 Fc Chimera Atto 488 (ATJ193) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa and 70-90 kDa, respectively.

Reconstitution Calculator

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, also known as Tumor Necrosis Factor Receptor Superfamily member 17 (gene name TNSFR17), is a member of the TNFR superfamily, due to the presence of its TNFR motif (1). BCMA is a type III membrane protein containing one extracellular cysteine rich domain, a transmembrane domain, and an intracellular domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily (2, 3). This binding to APRIL and BAFF has been shown to stimulate IgM production in peripheral B cells and increase the survival of cultured B cells (3, 4). This data suggests that BCMA may play an important role in B cell development, function, and regulation (5). BCMA expression has been found in immune organs and mature B cell lines (5). Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment (6). Within the ECD, human and mouse BCMA shares 62% amino acid identity. The expression of BCMA has also been linked to various cancers, autoimmune disorders, and infectious diseases (7). Proteolytic shedding of the BCMA extracellular domain generats soluble BCMA (sBCMA) via direct cleavage by gamma -secretase, and elevated sBCMA levels in serum may correlate with disease activity (8). More recently, BCMA has been indicated as a possible biomarker in various human immunological disease, and as a potential therapeutic target for multiple myeloma (MM) (9-11).

References

Hatzoglou, A. et al. (2000). J Immunol. 165:1322.
Schiemann, B. et al. (2001). Science. 293:2111.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Huang, H. et al. (2013). PNAS. 110:10928.
Laâbi, Y. et al. (1994). Nucleic Acids Res. 22:1147.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Coquery, C.M. et al. (2012) Crit Rev Immunol. 32:287.
Laurent, S.A. et al. (2015) Nat Commun. 6:7333.
Thaler, F.S. et al. (2017) Neuro Oncol. 19:1618.
Seckinger, A. et al. (2017) Cancer Cell. 31:396.
Lee, L. et al. (2018) Blood. 131:746.

Long Name

B Cell Maturation Factor

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17