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Recombinant Human Biglycan Protein, CF

Catalog #: 2667-CM
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2667-CM-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (11)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Biglycan Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the cell growth of mouse 3T3‑L1 mouse embryonic fibroblast adipose-like cells. Ward, M. and Ajuwon, K.M. (2011) Cell Proliferation 44:343. The ED50 for this effect is 1.5-6 μg/mL. Measured by its ability to increase the binding of BMP-4 to Chordin. Moreno, M. et al. (2005) EMBO J. 24:1397. 2-8 µg/mL of Recombinant Human Biglycan  will have a 50% maximum increase in the binding of recombinant human BMP-4 (0.1 µg/mL) to recombinant mouse Chordin (1 µg/mL).
Source
Mouse myeloma cell line, NS0-derived human Biglycan protein
Asp38-Lys368, with a C-terminal 6-His tag
Accession #
P21810
N-terminal Sequence
Analysis
Asp38
Predicted Molecular Mass
38 kDa
SDS-PAGE
40-45 kDa, reducing conditions

Product Datasheets

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2667-CM

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2667-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Biglycan

Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family. SLRP family members are characterized by N-terminal and C-terminal cysteine-rich domains that flank the central region containing 10-12 tandem leucine-rich repeats (LRRs). Biglycan function is important in the development and maintenance of many tissues (1). The human Biglycan cDNA encodes a 368 amino acid (aa) precursor with a 19 aa signal sequence and a 18 aa propeptide that is cleaved by BMP-1 (2). Mature human Biglycan shares 97% aa sequence identity with bovine, mouse, and rat Biglycan. The mature protein can be further cleaved by proteases, which compromises its inability to bind to other molecules (3, 4). The 45 kDa core protein is approximately one third the molecular weight of the fully glycanated form and can assemble into noncovalently-associated dimers (5). Biglycan binds, crosslinks, and stabilizes several proteins in the collagen matrix (6). It also binds and modulates the activity of a variety of non-matrix proteins, including C1q, collectins, TGF-beta, and Wnt3a (3, 7, 8). Biglycan binds to TLR2, TLR4, P2X4, and P2X7, leading to the increased production of inflammatory mediators, the migration of vascular endothelial cells, and the enhancement of oxidized LDL induced aortic valve calcification (9 - 11). Biglycan also blocks the uptake and degradation of acetylated LDL in atherosclerotic plaques by binding to SR-A (12). It stabilizes the neuromuscular junction through interactions with MuSK as well as alpha- and gamma-Sarcoglycan (13).

References
  1. Nastase, M.V. et al. (2012) J. Histochem. Cytochem. 60:963.
  2. Scott, I.C. et al. (2000) J. Biol. Chem. 275:30504.
  3. Boivin, W.A. et al. (2012) PLoS ONE 7:e33163.
  4. Monfort, J. et al. (2006) Arthritis Res. Ther. 8:R26.
  5. Scott, P.G. et al. (2006) J. Biol. Chem. 281:13324.
  6. Wiberg, C. et al. (2003) J. Biol. Chem. 278:37698.
  7. Groeneveld, T.W.L. et al. (2005) J. Immunol. 175:4715.
  8. Berendsen, A.D. et al. (2011) Proc. Natl. Acad. Sci. USA 108:17022.
  9. Babelova, A. et al. (2009) J. Biol. Chem. 284:24035.
  10. Yamamoto, K. et al. (2012) Br. J. Cancer 106:1214.
  11. Song, R. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:2711.
  12. Santiago-Garcia, J. et al. (2003) J. Biol. Chem. 278:6942.
  13. Amenta, A.R. et al. (2012) J. Neurosci. 32:2324.
    Entrez Gene IDs
    633 (Human); 12111 (Mouse)
    Alternate Names
    BGN; Biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A

    Citations for Recombinant Human Biglycan Protein, CF

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    11 Citations: Showing 1 - 10
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    1. Cystine-knot peptide inhibitors of HTRA1 bind to a cryptic pocket within the active site region
      Authors: Li, Y;Wei, Y;Ultsch, M;Li, W;Tang, W;Tombling, B;Gao, X;Dimitrova, Y;Gampe, C;Fuhrmann, J;Zhang, Y;Hannoush, RN;Kirchhofer, D;
      Nature communications
      Species: N/A
      Sample Types: Peptide, Recombinant Protein
      Applications: Bioassay
    2. Allosteric inhibition of HTRA1 activity by a conformational lock mechanism to treat age-related macular degeneration
      Authors: S Gerhardy, M Ultsch, W Tang, E Green, JK Holden, W Li, A Estevez, C Arthur, I Tom, A Rohou, D Kirchhofer
      Nature Communications, 2022-09-05;13(1):5222.
      Species: Human
      Sample Types: Recombinant Protein
      Applications: Bioassay
    3. The Proteoglycan Biglycan Modulates Platelet Adhesion and Thrombus Formation in a GPVI-Dependent Manner
      Authors: H Hoermann, I Krueger, N Maurus, F Reusswig, Y Sun, C Kohlmorgen, M Grandoch, JW Fischer, M Elvers
      International Journal of Molecular Sciences, 2021-11-10;22(22):.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    4. Toll-like receptor involvement in adolescent scoliotic facet joint degeneration
      Authors: DG Bisson, K Sheng, S Kocabas, E Krock, A Teles, N Saran, JA Ouellet, L Haglund
      J. Cell. Mol. Med., 2020-08-27;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    5. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
      Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
      Sci Rep, 2020-07-31;10(1):12912.
      Species: Snake
      Sample Types: Protein
      Applications: Bioassay
    6. Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells
      Authors: DA Ferraro, F Patella, S Zanivan, C Donato, N Aceto, M Giannotta, E Dejana, M Diepenbruc, G Christofor, M Buess
      BMC Cancer, 2019-04-04;19(1):312.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    7. Identification of the effector domain of biglycan that facilitates BMP-2 osteogenic function
      Authors: P Jongwattan, M Terajima, PA Miguez, W Querido, H Nagaoka, N Sumida, EG Gurysh, KM Ainslie, N Pleshko, L Perera, M Yamauchi
      Sci Rep, 2018-05-04;8(1):7022.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    8. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells
      Sci Rep, 2016-11-16;6(0):37128.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    9. L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro.
      Authors: Rzeniewicz K, Newe A, Rey Gallardo A, Davies J, Holt M, Patel A, Charras G, Stramer B, Molenaar C, Tedder T, Parsons M, Ivetic A
      Proc Natl Acad Sci U S A, 2015-03-09;112(12):E1461-70.
      Species: Human
      Sample Types: Cell Lysates
      Applications: Bioassay
    10. Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation.
      Authors: Roy A, Ganesh G, Sippola H, Bolin S, Sawesi O, Dagalv A, Schlenner S, Feyerabend T, Rodewald H, Kjellen L, Hellman L, Abrink M
      J Biol Chem, 2013-11-20;289(1):237-50.
      Species: Mouse
      Sample Types: Tissue Homogenates
      Applications: Bioassay

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