Recombinant Human BMP-8a (CHO-expressed) Protein, CF

Catalog #: 1073-BPC
1 Citation Datasheet / COA / SDS

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1073-BPC-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human BMP-8a (CHO-expressed) Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326. The ED₅₀ for this effect is 0.5-5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human BMP-8a protein
Ala264-His402

Accession #

AAP74559

N-terminal Sequence
Analysis

Ala264

Structure / Form

Disulfide-linked homodimer by SDS-PAGE under non-reducing conditions

Predicted Molecular Mass

15.7 kDa (monomer)

SDS-PAGE

19 kDa, reducing conditions

Product Datasheets

1073-BPC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1073-BPC

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BMP-8a

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7/OP-1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (2 - 4). BMP‑8a and BMP-8b, produced from separate genes, share 98% aa sequence identity in human but only 74% in mouse within the mature regions. Human BMP‑8a is synthesized as a 402 aa precursor protein that is cleaved between Arg263 and Ala264 to release the C-terminal mature protein. Mature human BMP‑8a shares 90% and 68% aa sequence identity with mouse mature BMP‑8a and -8b, respectively. BMP‑8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5 - 7). In the mouse it cooperates with BMP-7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP‑8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP‑8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10 - 12). BMP‑8a/b is also highly expressed in osteosarcomas (13).

References

Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
Chen, D. et al. (2004) Growth Factors 22:233.
Bragdon, B. et al. (2010) Cell Signal. Oct 16 epub.
Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
Zhao, G.-Q. et al. (1998) Development 125:1103.
Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
van der Horst, G. et al. (2002) Bone 31:661.
Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
Paic, F. et al. (2009) Bone 45:682.
Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.

Long Name

Bone Morphogenetic Protein 8a

Entrez Gene IDs

353500 (Human); 12163 (Mouse)

Alternate Names

BMP-8a; bone morphogenetic protein 8a; Op2; Osteogenic Protein 2

Related Research Areas

Citation for Recombinant Human BMP-8a (CHO-expressed) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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