Recombinant Human C-Reactive Protein/CRP Protein
Recombinant Human C-Reactive Protein/CRP Protein Summary
Product Specifications
Phe17-Pro224
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1707-CR
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and CaCl2 with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile 20 mM Tris-HCl, pH 8.0 containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1707-CR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and CaCl2. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile 20 mM Tris-HCl, pH 8.0. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: C-Reactive Protein/CRP
CRP is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are non-covalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively.
CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL-6, IL-1 and glucocorticoids are the major inducer of the CRP gene. In response to infection, inflammation or tissue damage, the level of CRP in human serum can increase 1,000-fold within 24-48 hours. It will come back to base level of less than 1 μg/mL very fast. Human CRP is an acute-phase serum protein that plays a role in the first line in host innate host defense. Like other pentraxins, CRP exhibits Ca++‑dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phogocytotic responses. In addition to phogocytosis, CRP also can induce production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF‑ alpha by monocytes. With these functions, human CRP is an important serum protein for anti-bacterial pathogen and clearance of damaged and apoptotic cells. However, in mouse, CRP is expressed at very low level and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is the major acute phase serum protein in mice. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.
- Gotschlich, E.C. and G.M. Edelman (1965) Proc. Natl. Acad. Sci. USA 54:558.
- Volanakis, J.E. (2001) Molecular Immunology 38:189.
- Bharadwaj, D. et al. (1999) J. Experimental Medicine 190:585.
- Ballou S.P. and G. Lozanski (1992) Cytokine 4:361.
- Danesh, J. et al. (2004) N. Engl. J. Med. 350:1387.
Citations for Recombinant Human C-Reactive Protein/CRP Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Recognition of highly branched N-glycans of the porcine whipworm by the immune system
Authors: Eckmair, B;Gao, C;Mehta, AY;Dutkiewicz, Z;Vanbeselaere, J;Cummings, RD;Paschinger, K;Wilson, IBH;
bioRxiv : the preprint server for biology
Species: N/A
Sample Types: Proteins
Applications: Bioassay -
Extending the dynamic range of biomarker quantification through molecular equalization
Authors: Newman, S;Wilson, B;Mamerow, D;Wollant, B;Nyein, H;Rosenberg-Hasson, Y;Maecker, H;Eisenstein, M;Soh, H;
bioRxiv
Species: Human
Sample Types: Serum
Applications: Bioassay -
Chromatin accessibility landscapes of immune cells in rheumatoid arthritis nominate monocytes in disease pathogenesis
Authors: D Zong, B Huang, Y Li, Y Lu, N Xiang, C Guo, Q Liu, Q Sha, P Du, Q Yu, W Zhang, P Cai, Y Sun, J Tao, X Li, S Cai, K Qu
Bmc Biology, 2021-04-16;19(1):79.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein
Authors: D Rho, S Kim
Biosensors, 2020-12-24;11(1):.
Species: Human
Sample Types:
Applications: Bioassay -
Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells
Authors: LA Henze, TTD Luong, B Boehme, J Masyout, MP Schneider, S Brachs, F Lang, B Pieske, A Pasch, KU Eckardt, J Voelkl, I Alesutan
Aging (Albany NY), 2019-08-03;11(15):5445-5462.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
C-Reactive Protein Promotes Diabetic Kidney Disease in db/db Mice via the CD32b-Smad3-mTOR signaling Pathway
Sci Rep, 2016-05-25;6(0):26740.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
Diabetologia, 2011-07-09;54(10):2713-23.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CRP enhances soluble LOX-1 release from macrophages by activating TNF-alpha converting enzyme.
Authors: Zhao XQ, Zhang MW, Wang F, Zhao YX, Li JJ, Wang XP, Bu PL, Yang JM, Liu XL, Zhang MX, Gao F, Zhang C, Zhang Y
J. Lipid Res., 2011-03-01;52(5):923-33.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
J. Leukoc. Biol., 2008-06-30;84(4):1109-19.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
C-reactive protein impairs human CD14+ monocyte-derived dendritic cell differentiation, maturation and function.
Authors: Zhang R, Becnel L, Li M, Chen C, Yao Q
Eur. J. Immunol., 2006-11-01;36(11):2993-3006.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
-
Is Recombinant Human C-Reactive Protein, Catalog # 1707-CR, a monomer or homopentamer?
This protein runs at approximately 26 kDa (monomer) under both reducing and non-reducing conditions on SDS-PAGE. However, from HPLC results, this protein will form a homopentamer, with a sole peak at 102-105kDa.
Reviews for Recombinant Human C-Reactive Protein/CRP Protein
Average Rating: 4.5 (Based on 2 Reviews)
Have you used Recombinant Human C-Reactive Protein/CRP Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
AF1707 as detection and MAB 17071 as capture antibody
The kits being tested were the Meso Scale Discovery V-Plex Vascualr Injury Panel 2 (human) kits.
