Recombinant Human C1qL3 Protein, CF

Catalog #: 9115-TN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9115-TN-050

Recombinant Human C1qL3 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human C1qL3 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.

Source

E. coli-derived human C1qL3 protein
Ser122 - Asp255, with an N-terminal Met and 6-His tag

Accession #

Q5VWW1

N-terminal Sequence
Analysis

Met

Structure / Form

Noncovalently-linked homotrimer.

Predicted Molecular Mass

16 kDa

SDS-PAGE

14 kDa, reducing conditions

Product Datasheets

9115-TN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9115-TN

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human C1qL3 (Catalog # 9115-TN) is coated at 2 µg/mL, Recombinant Human BAI2 Fc Chimera binds with a typical ED₅₀ of 25-150 ng/mL.

Reconstitution Calculator

Background: C1qL3

C1qTNF proteins constitute a highly conserved family of Acrp30/Adiponectin paralogs that share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a C-terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. C1qTNF3, also known as CORS26, is predominantly expressed in cartilage and is induced in mature adipocytes. C1qTNF10, also known as C1qL2, is a protein that shows 28% - 30% sequence identity with C1q subunits A, B, and C. It is predicted to contain one collagen-like and C1q domain. Mouse and human C1qTNF10 share over 90% amino acid sequence identity.

Long Name

Complement Component 1, Q Subcomponent-Like 3

Entrez Gene IDs

389941 (Human); 227580 (Mouse); 680404 (Rat)

Alternate Names

C1ql; C1qL3; C1q-like 3; C1qTNF13; Complement C1q-Like Protein 3; complement component 1, q subcomponent-like 3; CTRP13; K100