Recombinant Human C1qR1/CD93 Fc Chimera Avi-tag Protein, CF New

Catalog #: AVI2379 Datasheet / COA / SDS

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AVI2379-050

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Biotinylated Recombinant Human C1qR1/CD93 Fc Chimera Avi-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human C1qR1/CD93 Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human C1qR1/CD93 Fc Chimera Avi-tag binds to Human IGFBP-rp1/IGFBP-7 (K95R) Protein (Catalog # 1334-B7) with an ED₅₀ of 0.250-2.50 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human C1qR1/CD93 protein

Human C1qR (Ala24-Lys580) Accession # Q9NPY3.3	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

N-terminal Sequence
Analysis

Ala24

Structure / Form

Disulfide linked homodimer, Biotinylated via Avi-tag

Predicted Molecular Mass

87 kDa

SDS-PAGE

120-130 kDa, under reducing conditions.

Product Datasheets

AVI2379

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI2379

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human C1qR1/CD93 Fc Chimera Avi-tag Protein (Catalog # AVI2379) binds to Human IGFBP-rp1/IGFBP-7 (K95R) Protein (1334-B7) with an ED₅₀ of 0.250-2.50 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human C1qR1/CD93 Fc Chimera Avi-tag Protein (Catalog # AVI2379) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 120-130 kDa and 240-260 kDa, respectively.

Reconstitution Calculator

Background: C1qR1/CD93

C1qR1, also known as CD93 and C1qRp, is an approximately 125 kDa type-1 transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) containing C‑type lectin and EGF‑like domains, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, human CD93 shares 65% aa sequence identity with mouse and rat CD93. CD93 is expressed by vascular endothelial cells (5) and by a variety of hematopoietic cells (3‑9). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane (11‑13). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo (10, 15). Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (12, 14). CD93 plays a role in cardiovascular disease progression and modulates angiogenesis, inflammation and tumor growth and its interaction with insulin-like growth factor binding protein 7 (IGFBP7) contributes to abnormal tumor vasculature (16-17). Our Avi-tag Biotinylated Human C1qR1/CD93 Fc Chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
Nepomuceno, R.R. et al. (1997) Immunity 6:119.
Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.
Tossetta, G. et al. (2023) Cells 12:1778.
Sun, Y. et al. (2021) Sci. Transl. Med. 13:eabc8922.

Long Name

Complement Component C1q Receptor

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Alternate Names

AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4