Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF Summary
Product Specifications
Optimal concentration depends on cell type as well as the application or research objectives.
| Human Pro-Cadherin-12 (Gln24-Pro609) Accession # P55289 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2240-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate, NaCl and CaCl2. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Cadherin-12
The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin-12, also known as brain‑cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium‑dependent cell-cell adhesion through their five EC Ca++-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta -sheets that are oriented like bread in a sandwich. Human Cadherin‑12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6).
- Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
- Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
- Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
- Selig, S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2398.
- Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
- Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
Citations for Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Raludotatug Deruxtecan, a CDH6-Targeting Antibody-Drug Conjugate with a DNA Topoisomerase I Inhibitor DXd, is Efficacious in Human Ovarian and Kidney Cancer Models
Authors: Suzuki, H;Nagase, S;Saito, C;Takatsuka, A;Nagata, M;Honda, K;Kaneda, Y;Nishiya, Y;Honda, T;Ishizaka, T;Nakamura, K;Nakada, T;Abe, Y;Agatsuma, T;
Molecular cancer therapeutics
Species: N/A
Sample Types: Recombinant Protein
Applications: ELISA Capture -
Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid
Authors: Oldak, L;Lukaszewski, Z;Le?niewska, A;Go?awski, K;Lauda?ski, P;Gorodkiewicz, E;
International journal of molecular sciences
Species: Human
Sample Types: Peritoneal Fluid, Plasma
Applications: Surface Plasmon Resonance (SPR) -
Cadherin-11 promotes the metastasis of prostate cancer cells to bone.
Authors: Chu K, Cheng CJ, Ye X, Lee YC, Zurita AJ, Chen DT, Yu-Lee LY, Zhang S, Yeh ET, Hu MC, Logothetis CJ, Lin SH
Mol. Cancer Res., 2008-08-01;6(8):1259-67.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF and earn rewards!
Have you used Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
