Recombinant Human Casein Kinase 2 alpha Protein, CF

Catalog #: 7957-CK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7957-CK-020

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Casein Kinase 2 alpha Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Source

E. coli-derived human Casein Kinase 2 alpha protein
Asp253-Gln391, with an N-terminal Met and C-terminal 6-His tag

Accession #

P68400-1

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

14-16 kDa, reducing conditions

Product Datasheets

7957-CK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7957-CK

Formulation	Supplied as a 0.2 μm filtered solution in MES, NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Casein Kinase 2 alpha

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein (1). It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm (2). The kinase exists as a tetramer and is composed of an alpha, an alpha ', and two beta subunits (3). The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The Recombinant Human Casein Kinase 2 alpha contains amino acid 253 to 391 of the alpha subunit, which includes an O-GlcNAcylation site at Ser347 (4). O-GlcNAcylation of Casein Kinase 2 alpha not only regulates its kinase substrate specificity, but also regulates the stability of the protein. The Recombinant Human Casein Kinase 2 alpha is an ideal model protein for studying O-GlcNAcylation. It can be O-GlcNAcylated by OGT, the enzyme that introduces O-GlcNAc to target proteins. The introduced O-GlcNAc residue can be detected by Recombinant Human B3GALNT2 (Cat# 1848-GT), a GalNAc transferase that is highly active on O-GlcNAc (5).

References

Ahmad, K.A. et al. (2008) Adv. Enzyme Regul. 48:179.
Rabalski, A.J. et al. (2016) Clinical Cancer Research 22:2840.
Litchfield, D.W. (2003) Biochem. J. 369:1.
Tarrant, M.K. et al. (2012) Nat. Chem. Biol. 8:262.
Wu, Z.L. et al. (2018) Cell Chem. Biol. DOI:https://doi.org/10.1016/j.chembiol.2018.07.007.

Entrez Gene IDs

1457 (Human); 12995 (Mouse); 116549 (Rat)

Alternate Names

Casein Kinase 2 alpha; casein kinase 2, alpha 1 polypeptide; casein kinase II alpha 1 subunit; CK II alpha; CK2 catalytic subunit alpha; CK2A1; CK2A1casein kinase II subunit alpha; CKII; CSNK2A1; EC 2.7.11; EC 2.7.11.1; protein kinase CK2