Recombinant Human Caspr2 Protein, CF

Catalog #: 8207-CR
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8207-CR-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Caspr2 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human Caspr2 protein
Pro29-Ala1262, with a C-terminal 6-His tag

Accession #

Q9UHC6

N-terminal Sequence
Analysis

Pro29

Predicted Molecular Mass

138 kDa

SDS-PAGE

130-150 kDa, reducing conditions

Product Datasheets

8207-CR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8207-CR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Caspr2

Contact-associated Protein-like 2 (Caspr2) is a type I transmembrane member of the neurexin family of adhesion molecules (1). Human Caspr2 is the vertebrate homolog to Drosophila Neurexin IV, and the human and mouse Caspr2 orthologs share 94% amino acid sequence identity. Human Caspr2 contains two EGF-like domains, one F5/8 type C domain, one fibrinogen C-terminal domain, and four laminin G-like domains. It is highly expressed in neuronal tissue where it is primarily localized to the juxtaparanodal region of the axonal membrane (1, 2). Caspr2 acts in conjunction with 4.1B and Tag-1 as a scaffold that clusters Kv1 potassium channels at the juxtaparanodal region and is critical for axo-glial contacts (1-6). Caspr2 interacts with carboxypeptidase E in Golgi bodies during intracellular trafficking to the cell membrane (7). Caspr2 is also required for dendrite arborization and the normal development of neural networks (8). Mutations in Caspr2 are associated with predisposition to autism spectrum disorders, epilepsy, attention-deficit hyperactive disorder, and schizophrenia (8-11). The presence of autoantibodies against Caspr2 is associated with encephalitis, epilepsy, dysarthria, and paroxysmal kinesigenic dystonia (12-14). R&D Systems in-house testing indicates that Caspr2 can enhance bovine corneal endothelial cell adhesion.

References

Poliak, S. et al. (1999) Neuron 24:1037.
Poliak, S. et al. (2001) J. Neurosci. 21:7568.
Horresh, I. et al. (2010) J. Neurosci. 30:2480.
Traka, M. et al. (2003) J. Cell Biol. 162:1161.
Denisenko-Nehrbass, N. et al. (2003) Eur. J. Neurosci. 17:411.
Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Oiso, S. et al. (2009) J. Neurochem. 109:158.
Anderson, G.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:18120.
Penagarikano, O. and D.H. Geschwind (2012) Trends Mol. Med. 18:156.
Friedman, J.I. et al. (2008) Mol. Psychiatry 13:261.
Elia, J. et al. (2010) Mol. Psychiatry 15:637.
Lancaster, E. et al. (2011) Ann. Neurol. 69:303.
Balint, B. et al. (2013) J. Neurol. Sci. 327:73.
Krogias, C. et al. (2013) JAMA Neurol. 70:1056.

Long Name

Contactin Associated Protein-like 2

Entrez Gene IDs

26047 (Human); 66797 (Mouse)

Alternate Names

AUTS15; Caspr2; CASPR2DKFZp781D1846; CDFE; Cell recognition molecule Caspr2; CNTNAP2; contactin associated protein-like 2; contactin-associated protein 2; contactin-associated protein-like 2; homolog of Drosophila neurexin IV; KIAA0868; NRXN4; NRXN4Caspr2; PTHSL1

Related Research Areas

Citation for Recombinant Human Caspr2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1