Recombinant Human CCL18/PARC Protein Summary
Product Specifications
Ala21-Ala89
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
394-PA
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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394-PA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
|
Reconstitution Calculator
Background: CCL18/PARC
CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM‑CSF/IL‑4‑induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.
Citations for Recombinant Human CCL18/PARC Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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Structural basis of antibody inhibition and chemokine activation of the human CC chemokine receptor 8
Authors: Sun, D;Sun, Y;Janezic, E;Zhou, T;Johnson, M;Azumaya, C;Noreng, S;Chiu, C;Seki, A;Arenzana, TL;Nicoludis, JM;Shi, Y;Wang, B;Ho, H;Joshi, P;Tam, C;Payandeh, J;Comps-Agrar, L;Wang, J;Rutz, S;Koerber, JT;Masureel, M;
Nature communications
Species: Hamster
Sample Types: Transfected Whole Cells
Applications: Bioassay -
Adipose tissue specific CCL18 associates with cardiometabolic diseases in non-obese individuals implicating CD4+ T cells
Authors: N Subramania, K Hofwimmer, B Tavira, L Massier, DP Andersson, P Arner, J Laurenciki
Cardiovascular Diabetology, 2023-04-12;22(1):84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
Cell Reports, 2022-04-12;39(2):110670.
Species: Mouse
Sample Types: In Vivo, Tissue Culture Supernates
Applications: Bioassay, In Vivo -
Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD
Authors: K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch
International Journal of Molecular Sciences, 2021-07-02;22(13):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
Authors: L Amniai, C Ple, M Barrier, P de Nadai, P Marquillie, H Vorng, C Chenivesse, A Tsicopoulo, C Duez
International Journal of Molecular Sciences, 2021-04-09;22(8):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation
Authors: Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin
Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Osseous manifestations of adult Gaucher disease in the era of enzyme replacement therapy.
Authors: Deegan PB, Pavlova E, Tindall J
Medicine (Baltimore), 2011-01-01;90(1):52-60.
Applications: ELISA (Standard) -
Potential biomarkers of osteonecrosis in Gaucher disease.
Authors: Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM
Blood Cells Mol. Dis., 2010-11-13;46(1):27-33.
Applications: ELISA (Standard) -
CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
Immunology, 2009-12-02;129(4):536-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Novel antiviral activity of chemokines.
Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
Virology, 2006-04-17;350(2):484-92.
Species: Virus
Sample Types: Virus, Whole Cells
Applications: Binding Assay, Bioassay -
CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells.
Authors: Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM
J. Immunol., 2005-02-01;174(3):1723-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding.
Authors: Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP
Eur. J. Pharmacol., 2002-12-05;456(1):1-10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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We used this protein in an in-house ELISA along with mAb (394) and pAb (AF394) to detect CCL18 in human serum and plasma. This combination detected CCL18 in human serum and plasma efficiently and generated a good standard curve.
