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Recombinant Human CD157 Protein, CF

Catalog #: 4736-AC
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4736-AC-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD157 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260. The specific activity is >20 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human CD157 protein
Gly29-Lys292, with a C-terminal 6-His tag
Accession #
Q10588
N-terminal Sequence
Analysis
Gly29
Predicted Molecular Mass
31 kDa
SDS-PAGE
38 kDa and 40 kDa, reducing conditions

Product Datasheets

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4736-AC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4736-AC

Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 50 mM MES, 2.5 mM ZnCl2, pH 6.5
  • Recombinant Human CD157 (rhCD157) (Catalog # 4736-AC)
  • Substrate: Nicotinamide guanine dinucleotide (Sigma, Catalog # N-5131), 10 mM stock in deionized water
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhCD157 to 5 µg/mL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. Load into a black well plate 50 µL of 5 µg/mL rhCD157, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL Substrate.
  4. Incubate for 60 minutes at room temperature.
  5. Read at excitation and emission wavelengths of 300 nm and 410 nm (top read), respectively, in endpoint mode.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Fluorescence* (RFU) x Conversion Factor** (pmol/RFU)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard cyclic GDP ribose.

Per Well:
  • rhCD157: 0.25 µg
  • Substrate: 100 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD157

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA‑derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).

References
  1. Hussain, A. M. M. et al. (1998) Protein Express. Purif. 12:133.
  2. Sato, A. et al. (1999) Biochem. J. 337:491.
  3. Kaisho, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5325.
  4. Ortolan, E. et al. (2002) Cell Biochem. Funct. 20:309.
Long Name
Cluster of Differentiation 157
Entrez Gene IDs
683 (Human); 12182 (Mouse); 81506 (Rat)
Alternate Names
ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; bone marrow stromal cell antigen 1; BP-3; BST1; BST-1; cADPr hydrolase 2; CD157 antigen; CD157; Cyclic ADP-ribose hydrolase 2; EC 3.2.2.5; NAD(+) nucleosidase

Citations for Recombinant Human CD157 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. BST1 regulates nicotinamide riboside metabolism via its glycohydrolase and base-exchange activities
    Authors: K Yaku, S Palikhe, H Izumi, T Yoshida, K Hikosaka, F Hayat, M Karim, T Iqbal, Y Nitta, A Sato, ME Migaud, K Ishihara, H Mori, T Nakagawa
    Nature Communications, 2021-11-19;12(1):6767.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Binding of CD157 protein to fibronectin regulates cell adhesion and spreading.
    Authors: Morone S, Augeri S, Cuccioloni M, Mozzicafreddo M, Angeletti M, Lo Buono N, Giacomino A, Ortolan E, Funaro A
    J Biol Chem, 2014-04-21;289(22):15588-601.
    Species: Human
    Sample Types: N/A, Protein
    Applications: Bioassay, Western Blot

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