Recombinant Human CD163 Fc Chimera Protein, CF

Catalog #: 11228-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11228-CD-050

Recombinant Human CD163 Fc Chimera Protein Binding Activity.

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD163 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD163 Fc Chimera is immobilized at 2.00 μg/mL (100 μL/well), the concentration of human Haptoglobin/Hemoglobin (Hp/Hb) that produces 50% binding response is 20.0-250 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD163 protein

Human CD163 (Gly46-Ser1050) Accession # AAH51281.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAH51281.1

N-terminal Sequence
Analysis

Gly46

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

135 kDa

SDS-PAGE

145-160 kDa, under reducing conditions.

Product Datasheets

11228-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11228-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

Measured by its binding ability in a functional ELISA. When Recombinant Human CD163 Fc Chimera Protein (Catalog # 11228-CD) is immobilized at 2.00 μg/mL (100 μL/well), the concentration of human Haptoglobin/Hemoglobin (Hp/Hb) that produces 50% binding response is 20.0-250 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Human CD163 Fc Chimera Protein (Catalog # 11228-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-160 kDa and 290-320 kDa, respectively.

Reconstitution Calculator

Background: CD163

CD163, also known as M130, is a 130-160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1-3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and an 85 aa cytoplasmic region. The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (2). While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). Expression of CD163 is constitutive, and induced by glucocorticoids, IL10, IL6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocytederived dendritic cells (1, 6-7). In addition to clearing HbHp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNFlike weak inducer of apoptosis) and can function as an erythroblast adhesion receptor (4, 8, 10). The macrophage scavenger receptor CD163, which is upregulated in a number of inflammatory and malignant diseases, is a promising target on macrophages in anti-inflammatory and anti-cancer therapy in avoiding off-target effects on treatment of these diseases (11).

References

Sulahian, T.H. et al. (2000) Cytokine 12:1312.
Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
Austermann, J. et al. (2022) Cells 11:1979.
Kristiansen, M. et al. (2001) Nature 409:198.
Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
Schaer, D.J. et al. (2006) Blood 107:373.
Bover, L.C. et al. (2007) J. Immunol. 178:8183.
Fabriek, B.O. et al. (2007) Blood 109:5223.
Skytthe, M.K. et al. (2020) Int J. Mol. Sci. 21:5497.

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Alternate Names

CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163