Recombinant Human CD164 Fc Chimera Protein, CF

Catalog #: 9679-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9679-CD-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human CD164 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human CD164 protein

Human CD164 (Asp24-Thr160) Accession # Q04900-1	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q04900-1

N-terminal Sequence
Analysis

Asp24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa

SDS-PAGE

103-115 kDa, reducing conditions

Product Datasheets

9679-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9679-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD164

CD164 is a mucin-like glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells. It is composed of a signal sequence, an extracellular domain, a transmembrane domain and an intracellular domain (1). The CD164 cDNA encodes a 178-amino acid polypeptide core, of which residues 140‑164 represent the predicted transmembrane domain and residues 165-178 form a short cytoplasmic domain (2). Biochemical and functional analyses of human hematopoietic cell lines and transfectants demonstrate that CD164 comprises a homodimeric molecule of approximately 160 kDa. It plays an important role in mediating or regulating hematopoietic precursor cell adhesion to stroma, and may serve as a potent negative regulator of hematopoietic progenitor cell proliferation (3). Moreover, recent studies have indicated that CD164 may be a basophil activation marker, potentially useful in diagnosis of allergy caused by variety of allergens, including drug allergen (4). The molecule is extensively glycosylated with sites of O-linked glycosylation clustered in two discrete mucin domains separated by a more globular cysteine-rich region (1).

References