Recombinant Human CD200 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI2724 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI2724-050

Recombinant Human CD200 Fc Chimera Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD200 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD200R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CD200 Fc Chimera Avi-tag (Catalog # AVI2724) that produces 50% of the optimal binding response is 2-10 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD200 protein

Human CD200 (Gln31-Gly232) Accession # P41217	IEGRMDP	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P41217

N-terminal Sequence
Analysis

No results obtained: Gln31 inferred from enzymatic pyroglutamate treatment revealing Val32

Structure / Form

Disulfide-linked homodimer, Biotinylated via Avi-tag

Predicted Molecular Mass

51 kDa

SDS-PAGE

60-80 kDa, under reducing conditions

Product Datasheets

AVI2724

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI2724

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CD200R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD200 Fc Chimera Avi-tag (Catalog # AVI2724) binds with an ED₅₀ of 2-10 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD200 Fc Chimera Avi-tag Protein (Catalog # AVI2724)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa.

Reconstitution Calculator

Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5-7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor-ligand contact (6, 7, 10). In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14-16).

References

Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
Wright, G.J. et al. (2001) Immunology 102:173.
Shiratori, I. et al. (2005) J. Immunol. 175:4441.
Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
Fallarino, F. et al. (2004) J. Immunol. 173:3748.
Hoek, R.M. et al. (2000) Science 290:1768.
Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
Borriello, F. et al. (1997) J. Immunol. 158:4548.
Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
Hatherley, D. et al. (2005) J. Immunol. 175:2469.
Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
Cameron, C.M. et al. (2005) J. Virol. 79:6052.
Langlais, C.L. et al. (2006) J. Virol. 80:3098.

Entrez Gene IDs

4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)

Alternate Names

antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2