Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF

Catalog #: 8235-CD
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8235-CD-050

Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.7-3.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD2F-10/SLAMF9 protein

Human CD2F-10/SLAMF9 (Arg19-Ser210) Accession # Q96A28	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q96A28

N-terminal Sequence
Analysis

Arg19 & Val33

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

48 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Product Datasheets

8235-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8235-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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1 μg/lane of Recombinant Human CD2F/SLAMF9 Fc Chimera (Catalog # 8235-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 60-70 kDa and NR bands at 150-160 kDa.

Reconstitution Calculator

Background: CD2F-10/SLAMF9

The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F-10 protein, also known as SF2001 and CD85-H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F-10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co-receptors for lymphocyte activation and/or adhesion (1). Mature human CD2F-10 is a type I transmembrane glycoprotein that consists of a 218 amino acid (aa) extracellular domain (ECD) with one Ig-like C2-set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2-4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F-10 does not contain consensus immunoreceptor tyrosine-based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT-2 (1). Within aa 19-210, human CD2F-10 shares 57% and 59% aa sequence identity with mouse and rat CD2F-10, respectively. Alternative splicing of human CD2F-10 generates an isoform that lacks the Ig-like C2-set domain. CD2F-10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F-10 inhibits the secretion of IL-2 from activated human T cells.

References

Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
Fraser, C.C. et al. (2002) Immunogenetics 53:843.

Long Name

CD2 Family 10/SLAM Family Member 9

Entrez Gene IDs

89886 (Human); 98365 (Mouse)

Alternate Names

CD2 family member 10; CD2F10; CD2F-10; CD2F-10MGC88194; CD84 homolog 1; CD84H1; CD84-H1; CD84-H1cluster of differentiation 2 antigen family member 10; cluster of differentiation 84 homolog 1; SF2001; signaling lymphocytic activation molecule family member 2001; signaling lymphocytic activation molecule family member 9; SLAM family member 9; SLAMF9

Related Research Areas

Citation for Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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