Recombinant Human CD30/TNFRSF8 His-tag Avi-tag Protein, CF

Catalog #: AVI10239 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10239-050

Recombinant Human CD30/TNFRSF8 His-tag Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD30/TNFRSF8 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD30 Ligand/TNFSF8 (Catalog # 1028-CL) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human CD30/TNFRSF8 His-tag Avi-tag (Catalog # AVI10239) that produces 50% of the optimal binding response is 2-10 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD30/TNFRSF8 protein

Human CD30/TNFRSF8 (Phe19-Lys379) Accession # P28908.1	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

P28908.1

N-terminal Sequence
Analysis

Phe19

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

41 kDa

SDS-PAGE

82-98 kDa

Product Datasheets

AVI10239

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10239

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CD30 Ligand/TNFSF8 (Catalog # 1028-CL) is immobilized at 0.25 µg/mL, 100 µL/well, it binds Biotinylated Recombinant Human CD30/TNFRSF8 His-tag Avi-tag (Catalog # AVI10239) with an ED₅₀ of 2-10 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CD30/TNFRSF8 His-tag Avi-tag (Catalog # AVI10239) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 82-98 kDa.

Reconstitution Calculator

Background: CD30/TNFRSF8

CD30, also known as Ki-1 antigen and TNFRSF8, is a 120 kDa type I transmembrane glycoprotein belonging to the TNF receptor superfamily (1, 2). Mature human CD30 consists of a 361 amino acid (aa) extracellular domain (ECD) with six cysteine-rich repeats, a 28 aa transmembrane segment, and a 188 aa cytoplasmic domain (3). In contrast, mouse and rat CD30 lack 90 aa of the ECD and contain only three cysteine-rich repeats. Within common regions of the ECD, human CD30 shares 53% and 49% aa sequence identity with mouse and rat CD30, respectively. Alternate splicing of human CD30 generates an isoform that includes only the C‑terminal 132 aa of the cytoplasmic domain. CD30 is normally expressed on antigen-stimulated Th cells and B cells (4‑6). However, it is up‑regulated in Hodgkin’s disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (1, 5). CD30 signaling co‑stimulates antigen-induced Th0 and Th2 proliferation and cytokine secretion but favors a Th2-biased immune response (8). In the absence of antigenic stimulation, it can still induce T cell expression of IL‑13 (9). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4⁺CD8⁺ T cells (10, 11). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (5, 12). An 85‑90 kDa soluble form of CD30 is shed from the cell surface by TACE-mediated cleavage (13, 14). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (15).

References

Kennedy, M.K. et al. (2006) Immunology 118:143.
Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.
Durkop, H. et al. (1992) Cell 68:421.
Hamann, D. et al. (1996) J. Immunol. 156:1387.
Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147.
Gruss, H.-J. et al. (1994) Blood 83:2045.
Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174.
Del Prete, G. et al. (1995) J. Exp. Med. 182:1655.
Harlin, H. et al. (2002) J. Immunol. 169:2451.
Amakawa, R. et al. (1996) Cell 84:551.
Chiarle, R. et al. (1999) J. Immunol. 163:194.
Vinante, F. et al. (2002) Blood 99:52.
Hansen, H.P. et al. (1995) Int. J. Cancer 63:750.
Hansen, H.P. et al. (2000) J. Immunol. 165:6703.
Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.

Entrez Gene IDs

943 (Human); 21941 (Mouse); 25069 (Rat)

Alternate Names

CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8