Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

Catalog #: 10301-SR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10301-SR-050

Recombinant Human CD68/SR-D1 Fc Chimera Protein SDS-PAGE

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human S100A8 (Catalog # 9876-S8) is immobilized at 10 µg/mL (100 µL/well), it binds to Recombinant Human CD68/SR‑D1 Fc Chimera with an ED₅₀ of 5-30 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human CD68/SR-D1 protein

Human CD68/SR-D1 (Asn22-Ile320) Accession # P34810	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P34810

N-terminal Sequence
Analysis

Asn22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

58 kDa

SDS-PAGE

116-130 kDa, under reducing conditions

Product Datasheets

10301-SR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10301-SR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human CD68/SR-D1 Fc Chimera (Catalog # 10301-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 116-130 kDa and 230-260 kDa, respectively.

Reconstitution Calculator

Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family (1). Human CD68 contains 354 amino acids (aa), with a 21 aa signal sequence and a 298 aa extracellular domain (ECD), a 25 aa transmembrane domain, and a 10 aa cytoplasmic domain. The ECD of CD68 has 9 glycosylation sites and a mucin-like region in aa 23-140. Within the ECD, human CD68 shares a 65% and 67% amino acid sequence identity with that of mouse and rat CD68, respectively. CD68 is expressed on monocytes and tissue macrophages and serves as a scavenger receptor for oxidized LDL (2). In contrast to other members of the LAMP-family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appeared to have a more restricted, cell-type specific pattern of expression (3 ,4). CD68 is also a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (5, 6). CD68 influences the functions of cells through NF-kB/focal adhesion kinase pathway (7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity (8).

References

Holness, C.L., Simmons, D.L. (1993) Blood 81:1607.
Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453
Chistiakov, D. et al. (2016) Lab Invest. 97:13.
Bresnihan, B. et al. (2009) J Rheumatol. 36:1800.
Touati, M. et al. (2015) 56:332.
Wang, H. et al. (2014) Cancer Lett. 345:91.
Okada, K. et al. (2016) J. of Leukoc. Bio. 100:1093.

Entrez Gene IDs

968 (Human); 12514 (Mouse); 287435 (Rat)

Alternate Names

CD_antigen: CD68; CD68 antigen; CD68 molecule; CD68; DKFZp686M18236; gp110; LAMP4; macrophage antigen CD68; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1