Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

Catalog #: 9985-CM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9985-CM-050

Recombinant Human CEACAM-3/CD66d Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM‑3/CD66d Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED₅₀ of 0.05-0.4 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-3/CD66d protein

Human CEACAM-3 (Lys35-Gly155) Accession # P40198	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P40198

N-terminal Sequence
Analysis

Lys35

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

39.7 kDa

SDS-PAGE

48-54 kDa, reducing conditions

Product Datasheets

9985-CM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9985-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CEACAM-3/CD66d Fc Chimera (Catalog # 9985-CM) is immobilized at 1 µg/mL, Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED₅₀ of 0.05-0.4 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CEACAM‑3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-54 kDa and 96-110 kDa, respectively.

Reconstitution Calculator

Background: CEACAM-3/CD66d

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3 (CEACAM-3), or CD66d, is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Human CEACAM-3 is ~35 kDa, consisting of an extracellular domain (ECD) containing one IgV-like domain, a single transmembrane domain and a cytoplasmic tail. The cytoplasmic tail of CEACAM-3 contains an immunoreceptor tyrosine-based activation motif (ITAM), which recruits kinases to propagate pro-inflammatory signaling cascades (3). Interestingly, CEACAM-3 appears to be primate specific, with on non-primate orthologs currently identified (4). Originally discovered as a biomarker for colorectal cancer (5), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (6-8). Unlike other CEA family members, CEACAM-3 has not been shown to form cell–cell adhesion interactions with other CEACAM family members (9). CEACAM-3 has been found to be specifically expressed on human neutrophils and other granulocytes and appears to be a specific adaptation of the innate immune system to cope with a small set of host-specific pathogen (9). CEACAM-3 was identified as critical for opsonin-independent phagocytosis and bacterial clearance (10). CEACAM-3 binds to the colony opacity-associated (Opa) outer membrane proteins of bacteria, such as Neisseria gonorrhoeae, and triggers uptake of the pathogen and subsequent elimination (9, 10).

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