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Recombinant Human Cerebellin-2 Protein, CF

Catalog #: 7044-CB
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7044-CB-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Cerebellin-2 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Cerebellin-2 is coated at 5 μg/mL, biotinylated recombinant rat NRXN-1 beta binds with an apparent KD <1.5 nM. Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Cerebellin-2, immobilized at 3-30 μg/mL, is able to significantly enhance neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived human Cerebellin-2 protein
Arg50-Leu224, with an N-terminal HA (YPYDVPDYA) tag
Accession #
Q8IUK8
N-terminal Sequence
Analysis
Tyr
Predicted Molecular Mass
20 kDa
SDS-PAGE
25-35 kDa, reducing conditions

Product Datasheets

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7044-CB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7044-CB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cerebellin-2

Cerebellin-2 (CBLN2) is an approximately 25 kDa N-glycosylated protein in the Cerebellin family of TNF superfamily molecules (1). Cerebellins contain a collagenous domain and a C-terminal TNF/C1q-like domain. Human Cerebellin-2 consists of this 175 amino acid (aa) region following an N-terminal 28 aa potential cytoplasmic domain and a 21 aa potential transmembrane segment. Within the Cerebellin-like region, human Cerebellin-2 shares 99% aa sequence identity with mouse, rat, and chick Cerebellin-2 (2‑4). It is expressed in multiple regions of the nervous system including the neocortex, olfactory bulb, thalamus, hypothalamus, midbrain (5), preganglionic sensory nerves, dorsal root ganglia, and sensory and motoneurons of the spinal cord (4, 6) as well as in the zona glomerulosa of the adrenal cortex (7). Cerebellin-2 forms cell associated homo-oligomers and heteromers with Cerebellins-1, -3, and -4 [Iijima 1049]. It binds with high affinity to alpha and beta Neurexin 1 isoforms that contain the splice site 4 insert (+SS4) and more weakly to Neurexin 2 beta (+SS4) and Neurexin 3 beta (+SS4) (9, 10). These interactions promote presynaptic differentiation in cultures of cortical and cerebellar neurons (9, 10).

References
  1. Yuzaki, M. (2011) Curr. Opin. Neurobiol. 21:215.
  2. Kavety, B. et al. (1994) Brain Res. Mol. Brain Res. 27:152.
  3. Wada, C. and H. Ohtani (1991) Brain Res. Mol. Brain Res. 9:71.
  4. Yang, M. et al. (2010) J. Comp. Neurol. 518:2818.
  5. Miura, E. et al. (2006) Eur. J. Neurosci. 24:750.
  6. Reiner, A. et al. (2011) J. Comp. Neurol. 519:2225.
  7. Rucinski, M. et al. (2005) Int. J. Mol. Med. 15:411.
  8. Iijima, T. et al. (2007) Eur. J. Neurosci. 25:1049.
  9. Joo, J.-Y. et al. (2011) Biochem. Biophys. Res. Commun. 406:627.
  10. Matsuda, K. and M. Yuzaki (2011) Eur. J. Neurosci. 33:1447.
Entrez Gene IDs
147381 (Human); 12405 (Mouse); 291388 (Rat)
Alternate Names
CBLN2; cerebellin 2 precursor; Cerebellin2; Cerebellin-2

Citations for Recombinant Human Cerebellin-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Dysfunction of Glutamate Delta-1 Receptor-Cerebellin 1 Trans-Synaptic Signaling in the Central Amygdala in Chronic Pain
    Authors: PJ Gandhi, DY Gawande, GP Shelkar, SG Gakare, T Kiritoshi, G Ji, B Misra, R Pavuluri, J Liu, V Neugebauer, SM Dravid
    Cells, 2021-10-03;10(10):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Defining the ligand specificity of the deleted in colorectal cancer (DCC) receptor.
    Authors: Haddick P, Tom I, Luis E, Quinones G, Wranik B, Ramani S, Stephan J, Tessier-Lavigne M, Gonzalez L
    PLoS ONE, 2014-01-06;9(1):e84823.
    Applications: Surface Plasmon Resonance

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