Recombinant Human Chondrolectin His-tag Protein, CF

Catalog #: 11059-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11059-CL-100

Recombinant Human Chondrolectin His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Chondrolectin His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Chondrolectin His-tag Protein is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 binds with an ED₅₀ of 5.00-30.0 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human Chondrolectin protein
Arg22-Asn216, with C-terminal 6-His tag

Accession #

Q9H9P2.2

N-terminal Sequence
Analysis

Arg22

Predicted Molecular Mass

23 kDa

SDS-PAGE

34-40 kDa, under reducing conditions.

Product Datasheets

11059-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11059-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1.00 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) is immobilized at 1.00 ug/mL (100 µL/well), Recombinant Human IGFBP-5 (875-B5) binds with an ED₅₀ of 5.00󈛂.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Chondrolectin His-tag Protein (Catalog # 11059-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-40 kDa.

Reconstitution Calculator

Background: Chondrolectin

Chondrolectin (CHODL), also known as transmembrane protein MT75, is a type I transmembrane protein belonging to the C-type lectin-like domain superfamily. CHODL is predominantly expressed by the vascular muscle of the testis and smooth muscle of the heart and prostate (1) Mature CHODL consists of an extracellular domain (ECD) containing one C-type lectin carbohydrate recognition domain (CRD), a helical transmembrane domain, and a cytoplasmic tail. The ECD of CHODL shares 94% and 95% amino acid sequence identity with mouse and rat CHODL, respectively. Multiple isoforms of CHODL with varying C-terminal deletions are generated as a result of alternative splicing (2,3). CHODL is required for proper neuromuscular junction differentiation and is tightly regulated during early embryonic development (4). Dysregulation of CHODL expression is associated with spinal muscular atrophy in mouse (3). Overexpression of CHODL is associated with enhanced cell growth and invasion in lung cancer, while silencing of CHODL in colorectal cancer is a poor prognostic factor (5,6). Expression of specific CHODL isoforms is associated with T cell maturation (2).

References