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Recombinant Human Chromogranin A His-tag Protein, CF

Catalog #: 10422-CH Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10422-CH-050
Recombinant Human Chromogranin A His-tag Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Chromogranin A His-tag Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Human SCG3 His-tag (Catalog # 10374-SC) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Chromogranin A His‑tag (Catalog # 10422-CH) binds with an ED50 of 40‑360 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human Chromogranin A protein
Leu19-Gly457, with a C-terminal 6-His tag
Accession #
P10645.7
N-terminal Sequence
Analysis
Leu19
Predicted Molecular Mass
50 kDa
SDS-PAGE
60-80 kDa, under reducing conditions

Product Datasheets

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10422-CH

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10422-CH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Chromogranin A His-tag Protein Binding Activity View Larger

When Recombinant Human SCG3 His-tag (10374-SC) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Chromogranin A His-tag (10422-CH) binds with an ED50 of 40-360 ng/mL.

SDS-PAGE Recombinant Human Chromogranin A His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human Chromogranin A His-tag Protein (10422-CH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-80 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chromogranin A

Chromogranin A (CgA), also known as pituitary secretory protein I (SP-I), is a member of the granin family of regulated secretory proteins. CgA shares several protein characteristics common to the granin family: acidic isoelectric point, the capacity to bind calcium ions, the ability to form aggregates and multiple dibasic cleavage sites. Mature human CgA is 439 amino acids (aa) and contains 10 dibasic, proteolytic cleavage sites, capable of yielding several smaller peptides, each displaying a unique function (4). Mature human CgA shares 63% aa sequence identity with mouse and rat CgA. CgA is expressed exclusively in the secretory dense core granules of most normal and neoplastic neuroendocrine cells (1-3). Increased levels of CgA have been detected inpatients with neuroendocrine tumors as well as non-neuroendocrine tumors, hence CgA is an important serological marker for tumor diagnosis and monitoring tumorprogression/regression (5, 6). It has been demonstrated in mouse model that full-length CgA containing its C-terminal region can impair angiogenesis and tumor growth (5). In addition, CgA can bind to Secretogranin III to regulate the biogenesis of secretory granules (7).

References
  1. Bajetta, E. et al. (1999) Cancer 86:858.
  2. Taupenot, L. et al. (2003) N. Engl. J. Med. 348:1134.
  3. Modlin, I.M. et al. (2010) Am. Surg. Oncol. 17:2427.
  4. Marotta, V. et al. (2018) Endocr Relat Cancer 25:R11
  5. Curnis, F. et al. (2016) Oncotarget 7:72716.
  6. Gkolfinopoulos, S. et al. (2017) World J. Methodol. 7:9.
  7. Hosaka, M. et al. (2004) J Biol Chem 279:3627.
Entrez Gene IDs
1113 (Human); 12652 (Mouse); 24258 (Rat)
Alternate Names
betagranin (N-terminal fragment of chromogranin A); Betagranin; Catestatin; CGA; CHGA; Chromofungin; chromogranin A (parathyroid secretory protein 1); Chromogranin A; chromogranin-A; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I

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