Recombinant Human CLEC3B/Tetranectin Protein, CF

Catalog #: 5170-CL Datasheet / COA / SDS

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5170-CL-050

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Recombinant Human CLEC3B/Tetranectin Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When rhHGF (Catalog # 294-HGN) is immobilized at 5 µg/mL, the concentration of rhCLEC3B that produces 50% of the optimal binding response is found to be approximately 1‑5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human CLEC3B/Tetranectin protein
Glu22-Val202, with a C-terminal 6-His tag

Accession #

P05452

N-terminal Sequence
Analysis

Glu22

Predicted Molecular Mass

21.0 kDa (monomer)

SDS-PAGE

25 kDa, reducing conditions

Product Datasheets

5170-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5170-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CLEC3B/Tetranectin

CLEC3B (C‑type lectin domain family 3 member B), also known as Tetranectin, is a 17 kDa O‑glycosylated member of the C‑type lectin superfamily (1, 2). Mature human CLEC3B consists of an alpha -helical coiled-coil region followed by one C‑type lectin domain (CTLD) (3, 4). It shares 81% amino acid sequence identity with mouse and rat CLEC3B. CLEC3B associates into non‑covalent homotrimers, although it was named Tetranectin based on the proposal that it formed tetramers (4, 5). CLEC3B is secreted by endocrine, epithelial, endothelial, and mesenchymal cells including several hematopoietic cell types (6, 7). It shows binding selectivity for heparan sulfate, fucoidan, and chondroitin sulfates A, B, and C (8). CLEC3B binds the Kringle domain-containing proteins Plasminogen, tPA, and HGF, and it enhances the tPA-mediated activation of Plasminogen (5, 9). It also reduces the ability of Angiostatin (a Plasminogen fragment) to inhibit vascular endothelial cell proliferation (10). In mouse, CLEC3B is involved in the development and repair of muscle, spine, and skin (11 - 13). CLEC3B is upregulated in stromal cells surrounding various tumors but not in the tumor cells themselves (3, 14 ‑ 16). It is concentrated in the extracellular matrix at the leading edge of malignant tumors, a pattern that overlaps that of Plasminogen (3, 14, 16).

References

Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
Jaquinod, M. et al. (1999) Biol. Chem. 380:1307.
Wewer, U.M. and R. Albrechtsen (1992) Lab. Invest. 67:253.
Nielsen, B.B. et al. (1997) FEBS Lett. 412:388.
Clemmensen, I. et al. (1986) Eur. J. Biochem. 156:327.
Christensen, L. and I. Clemmensen (1989) Histochemistry 92:29.
Christensen, L. et al. (1987) Histochemistry 87:195.
Clemmensen, I. (1989) Scand. J. Lab. Invest. 49:719.
Westergaard, U.B. et al. (2003) Eur. J. Biochem. 270:1850.
Mogues, T. et al. (2004) J. Biomed. Biotechnol. 2:73.
Wewer, U. M. et al. (1998) Dev. Biol. 200:247.
Iba, K. et al. (2001) Mol. Cell. Biol. 21:7817.
Iba, K. et al. (2009) Wound Repair Regen. 17:108.
Obrist, P. et al. (2004) J. Clin. Pathol. 57:417.
Brunner, A. et al. (2007) Virchows Arch. 450:659.
De Vries, T.J. et al. (1996) J. Pathol. 179:260.

Long Name

C-type Lectin-like Receptor 3B

Entrez Gene IDs

7123 (Human); 21922 (Mouse)

Alternate Names

CLEC3B; C-type lectin domain family 3 member B; C-type lectin domain family 3, member B; DKFZp686H17246; Plasminogen kringle 4-binding protein; TETN; tetranectin (plasminogen binding protein); Tetranectin; TNA; TNAtetranectin (plasminogen-binding protein); TNtetranectin