Recombinant Human CLF-1/CLC Complex Protein Summary
Product Specifications
| Human CLF-1 (Ala38 - Arg422) Accession # O75462.1 |
10-His tag | |
| Human CLC (Leu28 - Phe225) Accession # Q9UBD9 |
6-His tag | |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1151-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1151-CL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CLF-1/CLC Complex
Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3), is a member of the IL-6 family of cytokines (1, 2). CLC associates with the secreted soluble cytokine-like factor1 (CLF-1), a member of the Cytokine Type I Receptor family, to form the heteromeric composite cytokine CLC/CLF-1 (3, 5). CLC can also form an alternate composite cytokine with soluble ciliary neurotrophic factor receptor alpha (CNTF R alpha ) (4). Co-expression of CLC with either CLF-1 or CNTF R alpha is required for the formation of the composite cytokines and for CLC secretion (3 - 5). CLC/CLF-1 binds to the membrane-associated CNTF R alpha to initiate the heterodimerization between gp130 and leukemia inhibitory factor receptor (LIFR) and stimulate thePI 3-kinase and the MAP kinase activity. The CLC/CLF-1 complex displays activities only on those cells expressing the functional tripartite receptor complex (5). Human and mouse CLF-1 share 96% amino acid sequence identity (3). Human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence homology. Human CLC also shares 96% amino acid sequence homology with mouse CLC. CLC/CLF-1 supports the survival of embryonic motor and sympathetic neurons and has been shown to induce astrocytes differentiation of fetal neuroepithelial cells (4 - 6). Both CLF-1 and CLC are expressed in the embryo, suggesting that the composite cytokine may have an important role in nervous system development (5, 7). CLC has also been shown to regulate immune functions by stimulating B cell proliferation and Ig production (8).
- Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
- Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132.
- Elson, G.C.A. et al. (1998) J. Immunol. 161: 1371.
- Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
- Elson, G.C.A. et al. (2000) Nature Neurosci. 3:867.
- Uemura, A. et al. (2002) Cytokine 18:1.
- Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
- Senaldi, G. et al. (2002) J. Immunol. 168:5690.
Citations for Recombinant Human CLF-1/CLC Complex Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Cytokine-like factor 1 gene expression is enriched in idiopathic pulmonary fibrosis and drives the accumulation of CD4+ T cells in murine lungs: evidence for an antifibrotic role in bleomycin injury.
Authors: Kass DJ, Yu G, Loh KS, Savir A, Borczuk A, Kahloon R, Juan-Guardela B, Deiuliis G, Tedrow J, Choi J, Richards T, Kaminski N, Greenberg SM
Am. J. Pathol., 2012-03-16;180(5):1963-78.
Species: Mouse, Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
Mol. Cell. Biol., 2010-06-28;17(0):4175.
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Surface Plasmon Resonance -
Novel regulators of kidney development from the tips of the ureteric bud.
Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002.
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay
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