Recombinant Human CNTFR/CNTF Complex Protein, CF

Catalog #: 10283-CC Datasheet / COA / SDS

Up to 100-fold greater activity than CNTF alone

Catalog #	Availability	Size / Price	Qty
10283-CC-020
10283-CC-050

Recombinant Human CNTFR/CNTF Complex Protein Bioactivity

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All CNTFR/CNTF Complex Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CNTFR/CNTF Complex Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.25-1.5 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CNTFR/CNTF Complex protein

Human CNTFR (Gln23-Ser342) Accession # P26992	GGGSGGGSGGGS	Human CNTF (Met1-Met200) Accession # P26441
N-terminus		C-terminus

Accession #

P26992 & P26441

N-terminal Sequence
Analysis

Gln23 inferred from deblocking revealing Arg24

Predicted Molecular Mass

59 kDa

SDS-PAGE

74-85 kDa, under reducing conditions

Product Datasheets

10283-CC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10283-CC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human CNTFR/CNTF Complex (Catalog # 10283-CC) stimulates proliferation of the TF-1 human erythroleukemic cell line. The ED₅₀ for this effect is 0.25-1.5 ng/mL. The Recombinant Human CNTFR/CNTF Complex has up to 100-fold greater activity than Recombinant CNTF alone (Catalog # 257-NT).

SDS-PAGE

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2 μg/lane of Recombinant Human CNTFR/CNTF Complex was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-85 kDa.

Reconstitution Calculator

Background: CNTFR/CNTF Complex

Human CNTF (ciliary neurotrophic factor) encodes a 200 amino acid polypeptide that lacks a signal sequence. It shares 83% and 84% sequence identity with the mouse and rat homologs, respectively. CNTF is known to be expressed by Schwann cells, astrocytes and T cells (1). CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes (2, 3). Engagement of CNTF with CNTFR alpha, LIFR, and gp130 on CD4 T cells has been shown to increase the number of IFN-gamma producing differentiated cells. IFN-gamma being a mediator of IFN-beta suggests CNTF's role in therapeutic effects of IFN-beta in multiple sclerosis (1, 4). CNTFR alpha contains a 22 amino acid signal peptide, 320 amino acids mature domain and a 30 amino acids propeptide. The mature human CNTFR alpha contains one Ig-like C2-type domain and two Fibronectin type III domains. CNTFR alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage (5). Mature human CNTFR alpha shares 98% and 96% sequence identity with its mouse and rat homologs in the mature domain, respectively. Similar to other GPI-linked proteins, soluble CNTFR alpha (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid (4). CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTFR alpha (4, 6).

References