Recombinant Human Collagen IV alpha 1/Arresten Protein, CF New

Catalog #: 11597-CL Datasheet / COA / SDS

His-tag

Catalog #	Availability	Size / Price	Qty
11597-CL-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Collagen IV alpha 1/Arresten Protein, CF Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human Collagen IV alpha 1 His-tag (Catalog # 11597-CL) binds Recombinant Human Integrin alpha 1 beta 1 (Catalog # 7064-AB) with an ED₅₀ of 85.0-850 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human Collagen IV alpha 1 protein
Ser1441-Thr1669, with a C-ter 6xHis tag

Accession #

P02462.3

N-terminal Sequence
Analysis

Ser 1441

Predicted Molecular Mass

26 kDa

SDS-PAGE

22-33 kDa, under reducing conditions

Product Datasheets

11597-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11597-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Human Collagen IV alpha 1 His-tag Protein (Catalog # 11597-CL) binds Recombinant Human Integrin alpha 1 beta 1 (7064-AB) with an ED₅₀ of 85.0‑850 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Collagen IV alpha 1 His-tag Protein (Catalog # 11597-CL) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 22‑33 kDa.

Reconstitution Calculator

Background: Collagen IV alpha 1

Type IV collagen is a member of the collagen superfamily. Unlike other collagens, type IV occurs only in basement membranes. In mammals, type IV collagen has up to 6 genetically distinct alpha chains, designated alpha-1(IV) to alpha-6(IV), each of which can form a triple helix structure with two other chains (1-3). Each chain contains three domains: a cysteine- and lysine-rich N-terminal domain, a collagenous triple repeat region, and a non-collagenous (NC1) C-terminal domain (4). The cysteine and lysine residues at the amino-terminal domain are essential for interchain crosslinking (5). Arresten, which comprise the C-terminal NC1 domain, has anti-angiogenic activity and inhibits tumor formation (6-7). Within this region, human Arresten shares 97% and 75% sequence identity with mouse and rat Arresten, respectively.

References

Myllyharju, J. and KI Kivirikko (2004) Trends Genet. 20:33.
Veit, G. et al. (2006) J. Biol. Chem. 281:3494.
Guey, S. and D. Herve (2022) Cereb. Circ. Cogn. Behav. 3:100140.
Hudson, B.G., et al. (2003) N. Engl. J. Med. 348:2543.
Than, M.E. et al. (2002) Proc. Natl. Acad. Sci. USA 99:6607.
Nyberg, P. et al. (2008) Exp. Cell Res. 314:3292.
Colorado, P.C. et al. (2000) Cancer Res. 60:2520.

Entrez Gene IDs

1282 (Human); 12826 (Mouse); 290905 (Rat)

Alternate Names

alpha-1 chain; Arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2