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Recombinant Human COMP His-tag Protein, CF

Catalog #: 3134-CPB
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3134-CPB-050
Recombinant Human COMP His-tag Protein Bioactivity
2 Images
Product Details
Citations (4)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human COMP His-tag Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Recombinant Human COMP/Thrombospondin‑5 immobilized at 10 µg/mL (100 µL/well) will induce more than 40% of ATDC-5 cell adhesion.
Source
Mouse myeloma cell line, NS0-derived human COMP/Thrombospondin-5 protein
Gln21-Ala757 (Ala256Arg), with a C-terminal 6-His tag
Accession #
P49747
N-terminal Sequence
Analysis
Gln21
Structure / Form
Disulfide linked homopentamer
Predicted Molecular Mass
81.8 kDa
SDS-PAGE
106-118 kDa, reducing conditions

Product Datasheets

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3134-CPB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3134-CPB

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human COMP His-tag Protein Bioactivity View Larger

Immobilized Recombinant Human COMP/Thrombospondin‑5 (Catalog # 3134-CPB) at 10 µg/mL (100 µL/well) induces more than 40% cell adhesion.

SDS-PAGE Recombinant Human COMP His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human COMP/Thrombospondin‑5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-118 kDa and 500 - 550 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous coiled‑coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin  alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type‑3 repeat, results in diminished calcium binding ability (13, 14).

References
  1. Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
  2. Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005.
  3. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
  4. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
  5. Newton, G. et al. (1994) Genomics, 24:435.
  6. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
  7. Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
  8. Ozbek, S. et al. (2002) EMBO J. 21:5960.
  9. Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655.
  10. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
  11. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
  12. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
  13. Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547.
  14. Hou, J. et al. (2000) Cell Calcium 27:309.
Long Name
Cartilage Oligomeric Matrix Protein
Entrez Gene IDs
1311 (Human); 12845 (Mouse)
Alternate Names
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5

Citations for Recombinant Human COMP His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP
    Authors: L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu
    J. Exp. Clin. Cancer Res., 2019-04-18;38(1):170.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways
    Authors: Q Li, C Wang, Y Wang, L Sun, Z Liu, L Wang, T Song, Y Yao, Q Liu, K Tu
    J. Exp. Clin. Cancer Res., 2018-09-19;37(1):231.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Molecular synergy in biolubrication: The role of cartilage oligomeric matrix protein (COMP) in surface-structuring of lubricin
    Authors: A Raj, M Wang, C Liu, L Ali, NG Karlsson, PM Claesson, A D?dinait?
    J Colloid Interface Sci, 2017-02-08;495(0):200-206.
    Applications: Bioassay
  4. Deficiency of Thrombospondin-4 in Mice Does Not Affect Skeletal Growth or Bone Mass Acquisition, but Causes a Transient Reduction of Articular Cartilage Thickness.
    Authors: Jeschke A, Bonitz M, Simon M, Peters S, Baum W, Schett G, Ruether W, Niemeier A, Schinke T, Amling M
    PLoS ONE, 2015-12-02;10(12):e0144272.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay

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