Recombinant Human CRELD2 Protein, CF

Catalog #: 3555-CR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3555-CR-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CRELD2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 1-5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human CRELD2 protein
Ala21-Leu353, with a C-terminal 6-His tag

Accession #

AAH50675

N-terminal Sequence
Analysis

Ala21

Predicted Molecular Mass

36.7 kDa

SDS-PAGE

55-60 kDa, reducing conditions

Product Datasheets

3555-CR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3555-CR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CRELD2

Cysteine-rich with EGF-like domain protein 2 (CRELD2) is an approximately 60 kDa glycoprotein that contains two EGF‑like domains and two FU domains (1). Mature human CRELD2 shares approximately 78% aa sequence identity with mouse and rat CRELD2. Alternative splicing of human CRELD2 generates additional isoforms with substitutions, deletions, and/or insertions in the C-terminal half of the molecule (2). The various isoforms are widely expressed in fetal and adult tissues (1‑3). CRELD2 localizes to the endoplasmic reticulum and Golgi and can also be secreted (3‑5). It is up‑regulated during the cellular stress‑induced unfolded protein response (UPR), cartilage and bone pathologies (MED and MCDS), and arsenic‑induced liver toxicity (4, 6‑8). CRELD2 interacts intracellularly with the alpha 4 and beta 2 subunits of the nicotinic acetylcholine receptor and inhibits cell surface expression of the receptor (3, 9). CRELD2 expression is up‑regulated in prostate cancer by dihydroxytestosterone and is down‑regulated following anti-androgen treatment (10).

References