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Recombinant Human CTLA-4 Fc Chimera His-tag Protein

Catalog #: 325-CT
7 Citations 5 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
325-CT-200/CF
325-CT-01M/CF

With Carrier

Catalog # Availability Size / Price Qty
325-CT-200
Recombinant Human CTLA-4 Fc Chimera His-tag Protein Bioactivity
2 Images
Product Details
Citations (7)
FAQs
Reviews (5)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CTLA-4 Fc Chimera His-tag Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED50 for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog #
140-B1) in the presence of PHA.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CTLA-4 protein
Human CTLA-4 (Ala37-Phe162)
Accession # Q6GR94		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
Q6GR94
N-terminal Sequence
Analysis
Ala37
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa (monomer)

Product Datasheets

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325-CT (with carrier)

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325-CT/CF (carrier free)

Product Datasheet
COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

325-CT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

325-CT/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CTLA-4 Fc Chimera His-tag Protein Bioactivity View Larger

Recombinant Human CTLA-4 Fc Chimera (Catalog # 325-CT) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7-1 Fc Chimera (Catalog # 140-B1) in the presence of PHA.

SDS-PAGE Recombinant Human CTLA-4 Fc Chimera His-tag Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human CTLA-4 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 51 kDa and 94 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CTLA-4

CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41‑43 kDa (2). Within the ECD, human CTLA-4 shares 68%, 71% and 83‑86% aa sequence identity with mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3‑5). Isoforms of 56‑79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1, 2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7‑1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11‑13).

References
  1. Harper, K. et al. (1991) J. Immunol. 147:1037.
  2. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65.
  3. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596.
  4. Tector, M. et al. (2009) BMC Immunol. 10:51.
  5. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015.
  6. Vijayakrishnan, L. et al. (2004) Immunity 20:563.
  7. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
  8. Jansson, A. et al. (2005) J. Immunol 175:1575.
  9. Darlington, P.J. et al. (2005) J. Immunol. 175:996.
  10. Platt, A.M. et al. (2010) J. Immunol. 185:1558.
  11. Wing, K. et al. (2008) Science 322:271.
  12. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421.
  13. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1524.
Long Name
Cytotoxic T-lymphocyte-associated Molecule 4
Entrez Gene IDs
1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
Alternate Names
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Citations for Recombinant Human CTLA-4 Fc Chimera His-tag Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
    Authors: BD Hock, SA MacPherson, JL McKenzie
    PLoS ONE, 2017-03-03;12(3):e0172858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3.
    Authors: Kowalczyk A, D'Souza C, Zhang L
    Eur J Immunol, 2014-01-22;44(4):1143-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers.
    Authors: Steidl C, Shah SP, Woolcock BW
    Nature, 2011-03-02;471(7338):377-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Human gammadelta T lymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement.
    Authors: Maniar A, Zhang X, Lin W, Gastman BR, Pauza CD, Strome SE, Chapoval AI
    Blood, 2010-06-02;116(10):1726-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009-10-28;1(4):4ra10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
    Authors: Sakthivel P, Shively V, Kakoulidou M
    J. Intern. Med., 2007-04-01;261(4):399-407.
    Applications: ELISA (Standard)
  7. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells.
    Authors: Lazetic S, Leong SR, Chang JC, Ong R, Dawes G, Punnonen J
    J. Biol. Chem., 2002-08-06;277(41):38660-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Recombinant Human CTLA-4 Fc Chimera His-tag Protein

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Recombinant Human CTLA-4 Fc Chimera His-tag Protein
By Anonymous on 09/12/2024
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human CTLA-4 Fc Chimera His-tag Protein 325-CT
Recombinant Human CTLA-4 Fc Chimera His-tag Protein
By Anonymous on 02/11/2019
Application: Binding assay/Protein-protein interaction

0.5 ug mCTLA-4-hIgG1 (Cat# 325-CT-200) was added to cells expressing mB7-1 GFP. Binding was detected with anti-human 594 secondary antibody.

Binding assay/Protein-protein interaction Recombinant Human CTLA-4 Fc Chimera His-tag Protein 325-CT
Recombinant Human CTLA-4 Fc Chimera His-tag Protein
By Anonymous on 11/02/2018
Application: Binding assay/Protein-protein interaction
Recombinant Human CTLA-4 Fc Chimera His Tagged Protein
By Anonymous on 03/24/2018
Application: Binding assay/Protein-protein interaction
Recombinant Human CTLA-4 Fc Chimera His Tagged Protein
By Kimberly Dickinson on 03/22/2018
Application: Binding assay/Protein-protein interaction
Reason for Rating: Works in our assay great!
Binding assay/Protein-protein interaction Recombinant Human CTLA-4 Fc Chimera His Tagged Protein 325-CT