Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF

Catalog #: E3-421 Datasheet / COA / SDS

His-tag

Catalog #	Availability	Size / Price	Qty
E3-421-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL2/RBX1 Complex protein
Met1 - Ala745 with a N-terminal 10-His Sumo tag (CUL2), Met1 - His108 (RBX1)

Accession #

Q13617.2 (CUL2), P62877.1 (RBX1)

Predicted Molecular Mass

102 kDa (neddylated CUL2), 12 kDa (RBX1)

Product Datasheets

E3-421

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-421

Formulation	Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: CUL2/RBX1 Complex

Cullin-2 is a core component of multiple cullin-RING based "ECS" (Elongin B/C-CUL2-SOCS-box protein) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in a variety of cellular processes. In the ECS complex, Cullin-2 serves as a scaffold that organizes the ELOB/C-associated recognition subunits with the RBX1 subunit and contributes to the catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.

References