Recombinant Human CXADR Fc Chimera Protein, CF Summary
Product Specifications
| Human CXADR (Leu20 - Gly237) Accession # P78310 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3336-CX
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: CXADR
CXADR (coxsackie and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein that belongs to the CTX family of the Ig superfamily (1 - 3). CXADR has received attention as a receptor that facilitates gene transfer mediated by most adenoviruses (1, 2). It is also an adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs (1, 2, 4). CXADR is essential for normal cardiac development in the mouse (7). It is expressed throughout brain neuroepithelium during development, but mainly in ependymal cells in the adult (4 - 6). The 365 amino acid (aa) human CXADR contains a 19 aa signal sequence, a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions (2). The fiber knob of adenoviruses attaches at a similar site, and evidence suggests that disruption of tight junctions facilitates virus binding (1, 2). The C-terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton (1, 8, 9). CXADR interaction with junctional adhesion molecule-like protein (JAML) has been shown to have important functional roles in immunity, inflammation, and tissue homeostatsis (10).The ECD of human CXADR shares 90% aa sequence identity with mouse, rat, and porcine CXADR, and 92% and 89% aa identity with bovine and canine CXADR, respectively. An alternately spliced isoform (CXADR2) that diverges in the C-terminal 15 aa shows a similar expression pattern (4, 11). Transcription of splice variants that produce soluble forms of CXADR has been detected, and secreted forms in serum and pleural fluid potentially block viral infection (12).
- Coyne, C.B. and J.M. Bergelson (2005) Adv. Drug Deliv. Rev. 57:869.
- Philipson, L. and R.F. Pettersson (2004) Curr. Top. Microbiol. Immunol. 273:87.
- Tomko, R.P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:3352.
- Raschperger, E. et al. (2006) Exp. Cell Res. 312:1566.
- Hotta, Y. et al. Dev. Brain Res. 143:1.
- Hauwel, M. et al. (2005) Brain Res. Rev. 48:265.
- Chen, J. et al. (2006) Circ. Res. 98:923.
- Fok, P.T. et al. (2007) J. Biol. Chem. 282:7512.
- Huang, K-C. et al. (2007) FEBS Lett. 581:2702.
- Verdino, P. et al. Science (2010) Science 329:1210.
- Mirza, M. et al. (2006) Exp. Cell Res. 312:817.
- Bernal, R.M. et al. (2002) Clin. Cancer Res. 8:1915.
Citations for Recombinant Human CXADR Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Nitric oxide suppression by secreted frizzled-related protein 2 drives retinoblastoma
Authors: P Jayabal, F Zhou, X Ma, KM Bondra, B Blackman, ST Weintraub, Y Chen, P Chévez-Bar, PJ Houghton, B Gallie, Y Shiio
Cell Reports, 2023-02-10;42(2):112103.
Species: N/A
Sample Types: Recombinant Proteins
Applications: Bioassay -
JAML immunotherapy targets recently activated tumor-infiltrating CD8+ T�cells
Authors: S Eschweiler, A Wang, C Ramírez-Su, A von Witzle, Y Li, SJ Chee, H Simon, M Mondal, M Ellis, GJ Thomas, V Chandra, CH Ottensmeie, P Vijayanand
Cell Reports, 2023-01-25;42(2):112040.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Heparan Sulfate Is a Cellular Receptor for Enteric Human Adenoviruses
Authors: A Rajan, E Palm, F Trulsson, S Mundigl, M Becker, BD Persson, L Frängsmyr, A Lenman
Viruses, 2021-02-14;13(2):.
Species: Human
Sample Types: Virus
Applications: Surface Plasmon Resonance -
Diversity within the adenovirus fiber knob hypervariable loops influences primary receptor interactions
Authors: AT Baker, A Greenshiel, L Coughlan, JA Davies, H Uusi-Kertt, DK Cole, PJ Rizkallah, AL Parker
Nat Commun, 2019-02-14;10(1):741.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Human adenovirus 52 uses sialic acid-containing glycoproteins and the coxsackie and adenovirus receptor for binding to target cells.
Authors: Lenman A, Liaci A, Liu Y, Ardahl C, Rajan A, Nilsson E, Bradford W, Kaeshammer L, Jones M, Frangsmyr L, Feizi T, Stehle T, Arnberg N
PLoS Pathog, 2015-02-12;11(2):e1004657.
Applications: Bioassay -
Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin.
Authors: Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS
PLoS ONE, 2011-03-11;6(3):e17787.
Applications: Bioassay
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Average Rating: 5 (Based on 3 Reviews)
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Reason for Rating: JAML binding detected via ELISA
