Recombinant Human CXCL9/MIG Protein Summary
Product Specifications
Thr23-Thr125
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
392-MG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
392-MG/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CXCL9/MIG
CXCL9, a member of the alpha subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T‑lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy‑terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy‑terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy‑terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes. The E. coli‑expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.
- Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
- Liao, F. et al. (1995) J. Exp. Med. 182:1301.
- Vanguri, P. (1995) J. Neuroimmunol. 56:35.
Citations for Recombinant Human CXCL9/MIG Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Distinct molecular and immune hallmarks of inflammatory arthritis induced by immune checkpoint inhibitors for cancer therapy
Authors: ST Kim, Y Chu, M Misoi, ME Suarez-Alm, JH Tayar, H Lu, M Buni, J Kramer, E Rodriguez, Z Hussain, SS Neelapu, J Wang, AY Shah, NM Tannir, MT Campbell, DL Gibbons, T Cascone, C Lu, GR Blumensche, M Altan, B Lim, V Valero, ME Loghin, J Tu, SN Westin, A Naing, G Garcia-Man, N Abdel-Waha, HA Tawbi, P Hwu, ICG Oliva, MA Davies, SP Patel, J Zou, A Futreal, A Diab, L Wang, R Nurieva
Nature Communications, 2022-04-12;13(1):1970.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CXCR4, but not CXCR3, drives CD8 T-cell entry into and migration through the murine bone marrow
Authors: M Goedhart, S Gessel, R van der Vo, E Slot, B Lucas, E Gielen, M Hoogenboez, T Rademakers, S Geerman, JD van Buul, S Huveneers, H Dolstra, G Anderson, C Voermans, MA Nolte
Eur. J. Immunol., 2019-02-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells
Authors: H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt
Oncol Lett, 2017-04-05;13(6):4224-4230.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
Invest Ophthalmol Vis Sci, 2015-01-20;56(2):1051-62.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
Authors: Badr G, Lefevre EA, Mohany M
PLoS ONE, 2011-09-01;6(9):e23741.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Chemokine receptor CXCR3 promotes growth of glioma.
Authors: Liu C, Luo D, Reynolds BA
Carcinogenesis, 2010-11-03;32(2):129-37.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Applications: ELISA (Standard) -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Applications: ELISA (Standard) -
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS, 2007;21(10):1263-72.
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard) -
Novel antiviral activity of chemokines.
Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
Virology, 2006-04-17;350(2):484-92.
Species: Virus
Sample Types: Virus, Whole Cells
Applications: Binding Assay, Bioassay -
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
J. Immunol., 2002-12-01;169(11):6515-21.
Applications: ELISA (Standard) -
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
J. Immunol., 2002-06-15;168(12):6244-52.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human CXCL9/MIG Protein
Average Rating: 5 (Based on 1 Review)
Have you used Recombinant Human CXCL9/MIG Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
MAB392 was used as a capture in a sandwich ELISA for MIG. The detection antibody was BAM392. The standard was 392-MG-010. Sensitivity was around 5 pg/ml.
