Recombinant Human DC-SIGN/CD209 Protein, CF

Catalog #: 9136-DC
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9136-DC-050

Recombinant Human DC-SIGN/CD209 Protein Bioactivity

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Product Details

Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DC-SIGN/CD209 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED₅₀ for this effect is 0.4-2 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human DC-SIGN/CD209 protein

YPYDVPDYA	Human DC-SIGN/CD209 (Gln59-Ala404) Accession # Q9NNX6
N-terminus	C-terminus

Accession #

Q9NNX6

N-terminal Sequence
Analysis

Tyr

Predicted Molecular Mass

41 kDa

SDS-PAGE

41-48 kDa, reducing conditions

Product Datasheets

9136-DC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9136-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Immobilized Recombinant Human DC-SIGN (Catalog # 9136-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED₅₀ for this effect is 0.4-2 μg/mL.

Reconstitution Calculator

Background: DC-SIGN/CD209

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (6, 7).

References

Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
Malcherek, G. et al. (2007) J. Immunol. 179:3804.
Geijtenbeek, T.B. et al. (2000) Cell 100:575.
Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Entrez Gene IDs

30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)

Alternate Names

CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Related Research Areas

Citations for Recombinant Human DC-SIGN/CD209 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
PLoS ONE, 2019-07-25;14(7):e0219480.
Species: Human
Sample Types: Natural Protein
Applications: Bioassay
Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins
Authors: C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge
FASEB J., 2018-01-29;0(0):fj201700619R.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay