Recombinant Human Di-Ub Non-hydrolyzable (K48) Agarose, CF

Name: Recombinant Human Di-Ub Non-hydrolyzable (K48) Agarose, CF
Brand: R&D Systems
SKU: UCN-202
Price: 251 USD

Catalog #: UCN-202 Datasheet / COA / SDS

Discontinued Product

UCN-202 has been discontinued.

View all Di-Ubiquitin products.

R&D Systems Recombinant Proteins and Enzymes

1 Image

All Di-Ubiquitin Proteins and Enzymes

Product Details

FAQs

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Di-Ub Non-hydrolyzable (K48) Agarose, CF Summary

Product Specifications

Activity

K48-linked Di-Ub (Ub2) Non-Hydrolyzable Chain Agarose is useful for the enrichment of known Ubiquitin chain-interacting proteins as well as the discovery of novel Ubiquitin chain-interacting proteins. We recommend equilibrating the resin by washing with 10 volumes of your desired aqueous buffer.

Source

E. coli-derived human Di-Ubiquitin protein

Each Ubiquitin contains a Pro substitution at position 73.

Accession #

P0CG47

Product Datasheets

UCN-202

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UCN-202

Formulation	100 μl of agarose supplied in a 200 μl total volume of 20% Ethanol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Do not freeze. 3 months from date of receipt, 2 to 8 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Di-Ubiquitin

With a predicted molecular weight of 17 kDa, Di-Ubiquitin is composed of two Ubiquitin monomers that are covalently linked through an isopeptide bond, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Ubiquitin has seven lysine residues that can participate in the formation of poly-Ubiquitin chains. The specific lysine residue used in Ubiquitin conjugation is thought to determine the function of poly-ubiquitination in cellular processes such as protein degradation, signaling, and trafficking (3-8).

Linkage specific, non-hydrolyzable di-Ubiquitin is resistant to the activity of deubiquitinating enzymes (DUB's) that cleave the isopeptide linkage between adjacent Ubiquitin molecules. It can be used to investigate binding interactions between di-Ubiquitin and proteins that contain elements such as Ubiquitin-associated domains (UBA's) or Ubiquitin-interacting motifs (UIM's). This product may also be useful in exploring the role of unanchored poly-Ubiquitin chains in some signaling pathways.

References