Recombinant Human DLL1 Fc Chimera Protein, CF

Catalog #: 10184-DL
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10184-DL-050

Recombinant Human DLL1 Fc Chimera Protein Bioactivity

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Product Details

Citations (2)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DLL1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED₅₀ for this effect is 0.1-0.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human DLL1 protein

Human DLL1 (Ser22-Glu537) Accession # O00548	IEGRMDP	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O00548

N-terminal Sequence
Analysis

Ser22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

82 kDa

SDS-PAGE

88-98 kDa, under reducing conditions

Product Datasheets

10184-DL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10184-DL

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human DLL1 Fc Chimera (Catalog # 10184-DL) enhances Recombinant Human BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED₅₀ for this effect is 0.1-0.5 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human DLL1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-98 kDa and 170-190 kDa, respectively.

Reconstitution Calculator

Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherent junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The up-regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also over-expressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13). Soluble DLL-1 was shown to inhibit differentiation of hematopoietic precursor cells (14).

References

Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
Karanu, F.N. et al. (2001) Blood 97:1960.
Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
Takahashi, Y. et al. (2003) Development 130:4259.
Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
Kiernan, A.E. et al. (2005) Development 132:4353.
Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
Limbourg, A. et al. (2007) Circ. Res. 100:363.
Purow, B.W. et al. (2005) Cancer Res. 65:2353.
Han. W. et al. (2000) Blood. 95(5):1616.

Long Name

Delta-like 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Alternate Names

delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

Related Research Areas

Citations for Recombinant Human DLL1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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DLL4-Notch3-WNT5B axis mediates bi-directional pro-metastatic crosstalk between melanoma and lymphatic endothelial cells
Authors: Alve, S;Gramolelli, S;Jukonen, J;Juteau, S;Pink, A;Manninen, AA;Hänninen, S;Monto, E;Lackman, MH;Carpén, O;Saharinen, P;Karaman, S;Vaahtomeri, K;Ojala, PM;
JCI insight
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells
Authors: K Matsumoto, V Kumar, S Varshney, AV Nairn, A Ito, F Pennarubia, KW Moremen, P Stanley, RS Haltiwange
The Journal of Biological Chemistry, 2022-05-25;0(0):102064.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay