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Recombinant Human E-Selectin/CD62E Protein, CF

Catalog #: ADP1
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
ADP1-050
ADP1-200
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Citations (12)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human E-Selectin/CD62E Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human E-Selectin coated plates (2 μg/mL with 100 μL/well), >80% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived human E-Selectin/CD62E protein
Accession #
P16581
Predicted Molecular Mass
58.6 kDa
SDS-PAGE
86-90 kDa, reducing conditions

Product Datasheets

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ADP1

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ADP1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation.

E‑Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T-cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E‑Selectin ligands on leukocytes. These interactions are labile, and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Citations for Recombinant Human E-Selectin/CD62E Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Neonatal neutrophils exhibit reduced NLRP3 inflammasome activation
    Authors: Wackerbarth, LM;Seifert, SB;Napoli, M;Rohwedder, I;Vogl, T;Scheiermann, C;Kolben, T;Nussbaum, C;Pruenster, M;Immler, R;Sperandio, M;
    Journal of leukocyte biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TRAIL-coated leukocytes to kill circulating tumor cells in the flowing blood from prostate cancer patients
    Authors: N Ortiz-Oter, JR Marshall, A Glenn, J Matloubieh, J Joseph, DM Sahasrabud, EM Messing, MR King
    BMC Cancer, 2021-08-06;21(1):898.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
    Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
    PLoS ONE, 2019-07-25;14(7):e0219480.
    Species: Human
    Sample Types: Natural Protein
    Applications: Bioassay
  4. Multivalent binding and biomimetic cell rolling improves the sensitivity and specificity of circulating tumor cell�capture
    Authors: JH Myung, MJ Eblan, JM Caster, S Park, MJ Poellmann, K Wang, JE Tepper, KA Tam, SM Miller, C Shen, RC Chen, T Zhang, B Chera, AZ Wang, S Hong
    Clin. Cancer Res., 2018-03-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Cholesterol Regulates Monocyte Rolling through CD44 Distribution
    Authors: AK Saha, P Osmulski, SF Dallo, M Gaczynska, TH Huang, AK Ramasubram
    Biophys. J., 2017-04-11;112(7):1481-1488.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  6. Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis.
    Authors: Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T
    Mol Ther, 2015-03-27;23(6):1044-54.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Bioassay
  7. c-FOS suppresses ovarian cancer progression by changing adhesion.
    Authors: Oliveira-Ferrer L, Rossler K, Haustein V, Schroder C, Wicklein D, Maltseva D, Khaustova N, Samatov T, Tonevitsky A, Mahner S, Janicke F, Schumacher U, Milde-Langosch K
    Br J Cancer, 2013-12-05;110(3):753-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. E-selectin mediated adhesion and migration of endothelial colony forming cells is enhanced by SDF-1alpha/CXCR4.
    Authors: Sun J, Li Y, Graziani G, Filion L, Allan D
    PLoS ONE, 2013-04-02;8(4):e60890.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Regulation of leukocyte recruitment by the long pentraxin PTX3.
    Authors: Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A
    Nat. Immunol., 2010-03-07;11(4):328-34.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  10. E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
    Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP
    Int. J. Cancer, 2008-10-15;123(8):1741-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Applications: ELISA (Standard)
  12. The role of endothelial cell reactive antibodies in peripheral arterial disease.
    Authors: Armitage JD, Lindsey NJ, Homer-Vanniasinkam S
    Eur J Vasc Endovasc Surg, 2005-10-14;31(2):170-5.
    Applications: ELISA (Standard)

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