Recombinant Human EBI3 Protein, CF

Catalog #: 11084-EI Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11084-EI-050

Recombinant Human EBI3 Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EBI3 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (Catalog # 7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human EBI3 protein
Arg21-Lys220

Accession #

Q14213.2

N-terminal Sequence
Analysis

Arg21

Predicted Molecular Mass

23 kDa

SDS-PAGE

30-40 kDa, under reducing conditions.

Product Datasheets

11084-EI

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11084-EI

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human EBI3 Protein (Catalog # 11084-EI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 kDa.

Reconstitution Calculator

Background: EBI3

EBI3 (Epstein-Barr virus-induced gene 3 protein) also known as IL-27B, is a secreted cytokine (1). It shares similar domain structure as IL-12B except there are only two fibronectin type -III domains instead of three. Through non-covalent interaction, EBI-3 binds to IL-12A (IL-12 p35), IL-23A (IL-23p19), or IL-27A (IL-27p28/IL-30) to form IL-35, IL-39, or IL-27, respectively (2-4). In addition to forming the heterodimeric cytokines, EBI-3 can also function on its own. Expression of EBI-3 is induced in neutrophils stimulated with TLR8 ligands, with no detectable increase in IL-35, IL-39 or IL-27 (5). EBI-3 also mediate IL-6 trans-signaling through receptor gp130 in B9 mouse plasmacytoma cells (6).

References

Devergne, O. et al. (1996) J. Virol. 70:1143.
Devergne, O. et al. (1997) Protc. Natl. Acad. Sci. U.S.A. 94:12041.
Pflanz, S. et al. (2002) Immunity 16:779.
Wang, X. et al. (2016) Eur. J. Immuno. 46:1343.
Cassatella, M.A. et al. (2020) J Leukoc. Biol. 108:1515.
Chehboun, S. et al. (2017) J Biol. Chem. 292:6644.

Long Name

Epstein-Barr virus induced gene 3

Entrez Gene IDs

10148 (Human); 50498 (Mouse); 680609 (Rat); 102144621 (Cynomolgus Monkey)

Alternate Names

cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 beta; IL-27 subunit beta; IL27B; IL-27B; IL35 EBI3; IL-35 EBI3; interleukin-27 subunit beta