Recombinant Human EDA2R/TNFRSF27/XEDAR His-tag Protein, CF

Catalog #: 11358-XD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11358-XD-050

Recombinant Human EDA2R/TNFRSF27/XEDAR His-tag Protein Binding Activity.

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All EDA2R/TNFRSF27/XEDAR Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EDA2R/TNFRSF27/XEDAR His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human EDA-A2/Ectodysplasin A2 (Catalog # 922-ED/CF) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human EDA2R/TNFRSF27/XEDAR His-tag (Catalog # 11358-XD) binds with an ED₅₀ of 40.0-500 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human EDA2R/TNFRSF27/XEDAR protein
Met1-Glu136, with a C-terminal 6-His tag

Accession #

NP_001229239.1

N-terminal Sequence
Analysis

Met1

Predicted Molecular Mass

16 kDa

SDS-PAGE

26-30 kDa, under reducing conditions.

Product Datasheets

11358-XD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11358-XD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human EDA-A2/Ectodysplasin A2 (922-ED/CF) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human EDA2R/TNFRSF27/XEDAR His-tag Protein (Catalog # 11358-XD) binds with an ED₅₀of 40.0‑500 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human EDA2R/TNFRSF27/XEDAR His-tag Protein (Catalog # 11358-XD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 26‑30 kDa.

Reconstitution Calculator

Background: EDA2R/TNFRSF27/XEDAR

X-linked Ectodysplasin Receptor (XEDAR), also known as EDA-2R and TNFRSF27, is an approximately 45 kDa transmembrane protein in the TNF receptor superfamily (1). Mature human XEDAR consists of a 136 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain (2). Within the ECD, human XEDAR shares 87% aa sequence identity with mouse and rat XEDAR. A 55 kDa long isoform of human XEDAR carries a 21 aa insertion in the juxtamembrane cytoplasmic domain (3). A 20 kDa fragment of the ECD can be shed by metalloprotease mediated cleavage (4). XEDAR binds selectively to the EDA-A2 variant of Ectodysplasin (EDA), while the closely related receptor EDAR binds selectively to the EDA-A1 variant (2). Other than a 2 aa deletion in its TNF-like domain, EDA-A2 is identical to EDA-A1 (2). Mutations in both EDAR and EDA are associated with hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and eccrine sweat gland morphogenesis (5). XEDAR itself is strongly associated with androgenetic alopecia (male hair loss) (6). XEDAR is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles (2, 7, 8). Even though it does not contain a cytoplasmic death domain, XEDAR can associate with Fas and induce EDA-A2 dependent apoptosis (7, 9). Its transcription is directly induced by p53, and XEDAR mediated cell death is p53 dependent (7, 10). XEDAR is down‑regulated in breast, colon, and lung cancers, particularly in cases with p53 mutations (7, 11). XEDAR also plays a role in EDA‑A2 induced skeletal muscle degeneration and osteoblast differentiation (8, 12).

References

Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
Yan, M. et al. (2000) Science 290:523.
Sinha, S.K. et al. (2002) J. Biol. Chem. 277:44953.
Tanikawa, C. et al. (2010) Mol. Cancer Res. 8:855.
Mikkola, M.L. (2009) Am. J. Med. Genet. 149A:2031.
Prodi, D.A. et al. (2008) J. Invest. Dermatol. 128:2268.
Tanikawa, C. et al. (2009) Oncogene 28:3081.
Newton, K. et al. (2004) Mol. Cell. Biol. 24:1608.
Sinha, S.K. and P.M. Chaudhary (2004) J. Biol. Chem. 279:41873.
Brosh, R. et al. (2010) FEBS Lett. 584:2473.
Punj, V. et al. (2010) Clin. Cancer Res. 16:1140.
Chang, B. et al. (2007) Cancer Gene Ther. 14:927.

Long Name

Ectodysplasin A2 Receptor

Entrez Gene IDs

60401 (Human); 245527 (Mouse); 710244 (Rhesus Macaque)

Alternate Names

ectodysplasin A2 receptor; EDA2R; EDA-A2 receptor; EDAA2R; EDA-A2R; EDAA2Rtumor necrosis factor receptor superfamily member XEDAR; EDAR2; TNFRSF27; XEDAR; XEDARTNFRSF27tumor necrosis factor receptor superfamily member 27; X-linked ectodysplasin-A2 receptor