Recombinant Human EDIL3 Protein, CF

R&D Systems | Catalog # 6046-ED

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human EDIL3 Protein (6046-ED)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

O43854

Applications

Bioactivity
View all EDIL3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human EDIL3 protein
Val17-Glu480 with a C-terminal 6x His

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val17, Asp24

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.
When 5 x 104 cells/well are added to Recombinant Human EDIL3 coated plates (5 µg/mL, 100 µL/well), >45% will adhere after 60 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

6046-ED
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EDIL3

EGF‑like repeat and discoidin I‑like domain‑containing protein 3 (EDIL3; also Del‑1 and integrin‑binding DEL1) is a 52 kDa extracellular matrix protein that is expressed by endothelial tissues during embryonic vascular development (1). Human EDIL3 is synthesized as a precursor with a 16 amino acid (aa) signal sequence and a 464 aa mature chain (SwissProt # O43854). The mature chain is composed of three epidermal growth factor (EGF) repeats and two discoidin‑I‑like domains (1). The second EGF repeat contains an RGD motif (1). Splicing variants produce two isoforms for human EDIL3. Isoform 2 has an A‑>G substitution at aa 66 and a deletion of
aa 67‑76 found in isoform 1. Mature human EDIL3 shares 96% aa sequence identity with mature mouse EDIL3. The RGD motif of EDIL3 binds alpha v beta 5 integrin, which, in turn, leads to increased angiogenic transcription factor HoxD3 expression (2). HoxD3activates alpha v beta 3 and uPA, resulting in the transformation of resting endothelial cells to an angiogenic state (2‑4). EDIL3 becomes quiescent at the time of birth, and is no longer expressed in normal adult tissues. It has been found, however,
re‑expressed in a number of human tumors as well as in ischemic muscles and ischemic brain tissue, which may play an important role in adult angiogenesis (2, 5‑6). EDIL3 promotes adherence and migration of endothelial cells, and acts as an endothelial cell survival agent through up‑regulation of Bcl‑2 expression (7). Exogenous application of EDIL3 has been demonstrated to augment angiogenesis and improve blood flow and tissue function in murine models of hind‑limb ischemia (6, 8), cardiac ischemia (9) and cerebral ischemia (2). EDIL3 has also been shown to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA‑1‑dependent leukocyte‑endothelial adhesion (10).

References

  1. Hidai, C. et al. (1998) Genes Dev. 12:21.
  2. Fan, Y. et al. (2008) Brain Res. 1219:1.
  3. Penta, K. et al. (1999) J. Biol. Chem. 274:11101.
  4. Rezaee, M. et al. (2002) Am. J. Physiol. Heart Circ. Physiol. 282:H1924.
  5. Aoka, Y. et al. (2002) Microvasc. Res. 64:148.
  6. Ho, H.K. et al. (2004) Circulation 109:1314.
  7. Pollman, M.J. et al. (1999) J. Cell Physiol. 178:359.
  8. Zhong, J. et al. (2003) J. Clin. Invest. 112:30.
  9. Kown, M.H. et al. (2003) Ann. Thorac. Surg. 76:1246.
  10. Choi, E.Y. et al. (2008) Science 322:1101.

Alternate Names

DEL1

Entrez Gene IDs

10085 (Human); 13612 (Mouse)

Gene Symbol

EDIL3

UniProt

O43854

Additional EDIL3 Products

Product Documents for Recombinant Human EDIL3 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human EDIL3 Protein, CF

For research use only

Citations for Recombinant Human EDIL3 Protein, CF

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