Recombinant Human EGFR Isoform vIII Protein, CF

Catalog #: 9565-ER
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9565-ER-050

Product Details

Citations (2)

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human EGFR Isoform vIII Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human EGFR protein
Leu25-Ser378 (Asn273Lys), with a C-terminal 6-His tag

Accession #

NP_001333870

N-terminal Sequence
Analysis

Leu25

Predicted Molecular Mass

39 kDa

SDS-PAGE

65 - 80 kDa, reducing conditions

Product Datasheets

9565-ER

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9565-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain (1-3). Human EGFR shares 90% identity with mouse and rat EGFR. The amino acid 25-378 of EGFR encodes the most common extracellular domain truncation mutant, termed EGFRvIII. It has been proved that EGFRvIII is relevant in a number of cancer types. EGFRvIII is tumor specific, and has not been found in normal tissues. EGFRvIII is highly tumorigenic, and can cause aggressive tumor phenotype, with increased invasion, proliferation, angiogenesis, and evasion of apoptosis. This EGFRvIII-mediated enhanced tumourigenicity combined with the lack of EGFRvIII expression in normal tissue makes it an ideal therapeutic targets (4).

References

Daly, R.J. (1999) Growth Factors, 16:255.
Schlessinger, J. (2000) Cell. 103:211.
Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
Kuan, C. T. et al. (2001) Endocrine-related cancer 8:83.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Related Research Areas

Citations for Recombinant Human EGFR Isoform vIII Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Heterodimerization of T cell engaging bispecific antibodies to enhance specificity against pancreatic ductal adenocarcinoma
Authors: Long, AW;Xu, H;Santich, BH;Guo, H;Hoseini, SS;de Stanchina, E;Cheung, NV;
Journal of hematology & oncology
Species: N/A
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
Visualizing Spatial and Stoichiometric Barriers to Bispecific T-cell Engager Efficacy
Authors: R You, J Artichoker, A Ray, H Gonzalez V, DA Rock, KP Conner, MF Krummel
Cancer Immunology Research, 2022-06-03;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Capture