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Recombinant Human ELFN2 Protein, CF

Catalog #: 9329-EF Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9329-EF-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ELFN2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons on a Laminin  alpha 4 coated plate. Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 300 ng of protein.
Source
Human embryonic kidney cell, HEK293-derived human ELFN2 protein
Asp23-Tyr397, with a C-terminal 6-His tag
Accession #
Q5R3F8
N-terminal Sequence
Analysis
Asp23
Predicted Molecular Mass
43 kDa
SDS-PAGE
71-94 kDa, non-reducing conditions

Product Datasheets

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9329-EF

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9329-EF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ELFN2

ELFN2 (Extracellular leucine-rich repeat and fibronectin type III domain-containing protein 2), also known as protein phosphatase 1 regulatory subunit 29, is a member of the extracellular Leucine-Rich Repeat superfamily (1). Expressed mainly in the nervous system, ELFN2 is a transmembrane protein that inhibits the activity of protein phosphatase 1 (PP1) complexes (2). Mature human ELFN2 consists of a 375 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 402 aa cytoplasmic tail. The ECD includes one fibronectin type-III domain, five leucine-rich repeats (LRR) and one LRR C-terminal (LRRCT) domain. Human ELFN2 shares 98% aa sequence identity with mouse and rat ELFN2. Expression of ELFN2 is quite restricted to the nervous system in which it is broadly expressed in the cortex in presumed glutamatergic neurons, in hippocampal pyramidal and granule cells, and in the striatum. These patterns are maintained in adults. Given the functions and discrete patterns of many known LRR family proteins it has been proposed that ELFN2 could provide the requisite specificity of cellular interactions to mediate a large number of selective connectivity decisions involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (1). Mutations of ELFN2 have been reported in pancreatic cancer radiation resistance (3) and stress fracture (4).

References
  1. Dolan J. et al. (2007) BMC Genomics 8:320.
  2. Hendrickx, A. et al. (2009) Chem. Biol. 16:365.
  3. Friedman E. et al. (2014) Genet Res (Camb) 96:e004.
  4. Souchek L.L. et al. (2014) British Journal of Cancer 111:1139.
Long Name
Extracellular Leucine-Rich Repeat And Fibronectin Type III Domain Containing 2
Entrez Gene IDs
114794 (Human); 207393 (Mouse)
Alternate Names
dJ63G5.3 (putative Leucine rich protein); dJ63G5.3; ELFN2; extracellular leucine-rich repeat and fibronectin type III containing 2; extracellular leucine-rich repeat and fibronectin type III domain containing 2; extracellular leucine-rich repeat and fibronectin type III domain-containing protein 2; KIAA1904; leucine rich repeat containing 62; leucine-rich repeat and fibronectin type-III domain-containing protein 6; leucine-rich repeat-containing protein 62; LRRC62; PPP1R29; protein phosphatase 1, regulatory subunit 29

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