Recombinant Human Embigin/EMB Fc Chimera Protein CF

Catalog #: 10832-EM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10832-EM-050

Recombinant Human Embigin/EMB Fc Chimera Protein SDS-PAGE.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Embigin/EMB Fc Chimera Protein CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Embigin/EMB Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Source

Human embryonic kidney cell, HEK293-derived human Embigin/EMB protein

Human Embigin/EMB (Asp33-Pro260) Accession # Q6PCB8.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q6PCB8.1

N-terminal Sequence
Analysis

Asp33

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

52 kDa

SDS-PAGE

85-100 kDa, under reducing conditions

Product Datasheets

10832-EM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10832-EM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human Embigin/EMB Fc Chimera (Catalog # 10832-EM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa and 170-200 kDa, respectively.

Reconstitution Calculator

Background: Embigin/EMB

Embigin (EMB) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily (IgSF) proteins. It is a cell adhesion molecule that is preferentially expressed during mouse embryogenesis (1). Embigin, along with basigin and neuroplastin, form a small subgroup within the IgSF, with all members possessing a glutamate at the same position in the transmembrane domain (2). Mature, human Embigin is highly glycosylated and consists of an extracellular domain (ECD) wjth two Ig domains, a transmembrane region, and a short intracellular region. Within the ECD, human EMB shares 75% and 62% amino acid identity with mouse and rat EMB, respectively. Embigin is an accessory protein of monocarboxylic acid transporter, MCT2 which participates in transporting lactic acid between glial cells and neurons (3). Thus, it may be related to brain energy metabolism and has been investigated as a susceptible gene for schizophrenia (4). Embigin is highly expressed in early embryos of mice, and in the heart, lung, brain and other tissues of adult rats (5, 6). Loss of embigin promotes proliferation, anchorage-independent growth, and migration ability of breast cancer cells (7).

References