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Recombinant Human EphA3 Fc Chimera Protein, CF

Catalog #: 6444-A3
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6444-A3-050
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EphA3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human (rh) EphA3 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed rhEphrin A5 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 5 ‑ 25 ng/mL.

Source
Mouse myeloma cell line, NS0-derived human EphA3 protein
Human EphA3
(Met1 - Gln541)
Accession # NP_005224
 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
NP_005224
N-terminal Sequence
Analysis
Glu21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.4 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions

Product Datasheets

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6444-A3

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6444-A3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA3

EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a 135 kDa glycosylated member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA3 preferentially binds to Ephrin-A5. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). The 521 amino acid (aa) extracellular domain (ECD) of human EphA3 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type II domains. The 418 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (3, 4). Within the ECD, human EphA3 shares 96% aa sequence identity with mouse and rat EphA3. Alternate splicing generates a secreted isoform that consists of nearly the entire ECD. EphA3 is expressed in the developing forebrain, retinal axons, some spinal cord motor neurons, and the heart where it plays an important role in axonal repulsion and organ morphogenesis (5 - 8). It is upregulated on some hematopoietic and solid tumor cells and on astrocytes surrounding injured nervous tissue (3, 5, 9 - 11). EphA3 ligation inhibits cellular adhesion to fibronectin as well as cellular migration (9, 10). Transmembrane EphA3 associates in cis with ADAM10 which then promotes the cleavage in trans of Ephrin-A5 (12). It also associates in cis with Ephrin-A5 on retinal axons, thereby preventing the activation of EphA3 by Ephrin-A (13). Multiple tyrosine residues within the cytoplasmic region of EphA3 become phosphorylated during ligand-induced signaling (14, 15).

References
  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Wicks, I.P. et al. (1992) Proc. Natl. Acad. Sci. 89:1611.
  4. Lackmann, M. et al. (1998) J. Biol. Chem. 273:20228.
  5. Chiari, R. et al. (2000) Cancer Res. 60:4855.
  6. Kudo, C. et al. (2005) J. Comp. Neurol. 487:255.
  7. Kilpatrick, T.J. et al. (1996) Mol. Cell. Neurosci. 7:62.
  8. Stephen, L.J. et al. (2007) Dev. Biol. 302:66.
  9. Smith, L.M. et al. (2004) Exp. Cell Res. 292:295.
  10. Clifford, N. et al. (2008) J. Cell. Biochem. 105:1250.
  11. Irizarry-Ramirez, M. et al. (2005) J. Neurotrauma 22:929.
  12. Janes, P.W. et al. (2005) Cell 123:291.
  13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  14. Shi, G. et al. (2010) Cell Res. June epub.
  15. Hu, T. et al. (2009) Biochemistry 48:6369.
Long Name
Eph Receptor A3
Entrez Gene IDs
2042 (Human); 13837 (Mouse)
Alternate Names
Cek4; EC 2.7.10; EC 2.7.10.1; EK4; EPH receptor A3; EphA3; EPH-like kinase 4; ephrin type-A receptor 3; ETK; Hek4; HEKETK1eph-like tyrosine kinase 1; human embryo kinase 1; TYRO4 protein tyrosine kinase; Tyro4; Tyrosine-protein kinase receptor ETK1; Tyrosine-protein kinase TYRO4

Citations for Recombinant Human EphA3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022-03-10;0(0):101817.
    Species: N/A
    Sample Types: Protein
    Applications: ELISA Capture
  2. IL-26 mediates epidermal growth factor receptor-tyrosine kinase inhibitor resistance through endoplasmic reticulum stress signaling pathway in triple-negative breast cancer cells
    Authors: T Itoh, R Hatano, Y Horimoto, T Yamada, D Song, H Otsuka, Y Shirakawa, S Mastuoka, N Iwao, TM Aune, NH Dang, Y Kaneko, K Okumura, C Morimoto, K Ohnuma
    Cell Death & Disease, 2021-05-21;12(6):520.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
    Nat Commun, 2016-05-05;7(0):11473.
    Species: Human
    Sample Types: Recombinant Protein

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