Recombinant Human Ephrin-A3 Fc Chimera Biotinylated Protein
Recombinant Human Ephrin-A3 Fc Chimera Biotinylated Protein Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
| Human Ephrin-A3 (Asn31 - Ser209) Accession # AAA52368 | IEGRMD | Human IgG1 (Pro100 - Lys330) | 6-His tag |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
BT359
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL with sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ephrin-A3
Ephrin‑A3, also known as EHK1‑L, EFL‑2, and LERK‑3, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin‑A3 preferentially interacts with receptors in the EphA family (1, 2). Ephrin‑A3 is an unusual Ephrin‑A molecule in its dependence on heparan sulfate binding for full activity (3). Mature human Ephrin‑A3 shares 92% aa sequence identity with mouse and rat Ephrin‑A3 (4). Its expression is restricted to discreet locations during the early development of multiple tissues (5). Ephrin‑A3 expression can be up‑ or down‑regulated by hypoxia in the hippocampus or vascular endothelial cells, respectively (6, 7). Ephrin‑A3 down‑regulation contributes to hypoxia‑induced endothelial cell chemotaxis, proliferation, and tubule formation (7). Its interaction with EphA receptors induces neurite growth cone collapse and the repulsion of migrating axons (8 ‑ 10). This activity is important for the accurate pathfinding of migrating axons during CNS development (10). Astrocyte‑expressed Ephrin‑A3 activates EphA4 on hippocampal neurons to regulate dendritic spine morphology and long term potentiation (8, 11, 12). The same interaction induces reverse signaling through Ephrin‑A3 to regulate glutamate uptake by the astrocyte and the availability of glutamate in the synapse (11, 12). Astrocyte‑expressed Ephrin‑A3 also interacts with EphA7 to inhibit the proliferation of neural progenitor cells (13).
- Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
- Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
- Irie, F. et al. (2008) Proc. Natl. Acad. Sci. 105:12307.
- Davis, S. et al. (1994) Science 266:816.
- Duffy, S.L. et al. (2006) Gene Expr. Patterns 6:719.
- Pulkkinen, K. et al. (2008) FEBS Lett. 582:2397.
- Fasanaro, P. et al. (2008) J. Biol. Chem. 283:15878.
- Murai, K.K. et al. (2003) Nat. Neurosci. 6:153.
- Stein, E. et al. (1999) J. Neurosci. 19:8885.
- Rudolph, J. et al. (2010) Cell Adh. Migr. 4:400.
- Filosa, A. et al. (2009) Nat. Neurosci. 12:1285.
- Carmona, M.A. et al. (2009) Proc. Natl. Acad. Sci. 106:12524.
- Jiao, J. et al. (2008) Proc. Natl. Acad. Sci. 105:8778.
Citation for Recombinant Human Ephrin-A3 Fc Chimera Biotinylated Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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