Home / Ephrin-B1 / Recombinant Human Ephrin-B1 Fc Chimera Protein, CF

Recombinant Human Ephrin-B1 Fc Chimera Protein, CF

Catalog #: 7654-EB
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7654-EB-050
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Citations (5)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Ephrin-B1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse EphB3 Fc Chimera (Catalog # 432-B3) is coated at 2 μg/mL, Recombinant Human Ephrin-B1 Fc Chimera binds with an apparent KD <0.2 nM. 
Source
Mouse myeloma cell line, NS0-derived human Ephrin-B1 protein
Human Ephrin-B1
(Leu28-Ser236)
Accession # P98172		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
P98172
N-terminal Sequence
Analysis
Leu28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.5 kDa (monomer)
SDS-PAGE
62-67 kDa, reducing conditions

Product Datasheets

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7654-EB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7654-EB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-B1

Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature human Ephrin-B1 consists of a 210 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, human Ephrin-B1 shares approximately 94% aa sequence identity with mouse and rat Ephrin-B1. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up‑regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Davis, S. et al. (1994) Science 266:816.
  4. Beckmann, M.P. et al. (1994) EMBO J. 13:3757.
  5. Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
  6. Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
  7. Tanaka, M. et al. (2005) EMBO J. 24:3700.
  8. Genet, G. et al. (2012) Circ. Res. 110:688.
  9. Hashimoto, T. et al. (2007) Kidney Int. 72:954.
  10. Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
  11. Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
  12. Cheng, S. et al. (2012) PLoS ONE 7:e32887.
  13. Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
  14. Davy, A. et al. (2004) Genes Dev. 18:572.
  15. Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
  16. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
  17. Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.
Entrez Gene IDs
1947 (Human); 13641 (Mouse); 25186 (Rat)
Alternate Names
Cek5-L; EFL3; EFL-3; EFNB1; ELK ligand; ELK-L; EphrinB1; Ephrin-B1; LERK-2; STRA-1

Citations for Recombinant Human Ephrin-B1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands
    Authors: D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils
    J. Leukoc. Biol., 2020-04-26;0(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  2. The Shb scaffold binds the Nck adaptor protein, p120 RasGAP and Chimaerins and thereby facilitates heterotypic cell segregation by the receptor EphB2
    Authors: MJ Wagner, MS Hsiung, GD Gish, RD Bagshaw, SA Doodnauth, MA Soliman, C Jørgensen, M Tucholska, R Rottapel
    J. Biol. Chem., 2020-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Using enhanced number and brightness to measure protein oligomerization dynamics in live cells
    Authors: F Cutrale, D Rodriguez, V Hortigüela, CL Chiu, J Otterstrom, S Mieruszyns, A Seriola, E Larrañaga, A Raya, M Lakadamyal, SE Fraser, E Martinez, S Ojosnegros
    Nat Protoc, 2019-02-01;14(2):616-638.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The osteoprogenitor-specific loss of ephrinB1 results in an osteoporotic phenotype affecting the balance between bone formation and resorption
    Authors: A Arthur, TM Nguyen, S Paton, A Klisuric, ACW Zannettino, S Gronthos
    Sci Rep, 2018-08-24;8(1):12756.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Blocking the Interaction between EphB2 and ADDLs by a Small Peptide Rescues Impaired Synaptic Plasticity and Memory Deficits in a Mouse Model of Alzheimer's Disease
    Authors: Can Gao
    J. Neurosci., 2016-11-23;36(47):11959-11973.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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