Recombinant Human ERMAP Fc Chimera Protein, CF

Recombinant Human ERMAP Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: ERMAP

Erythroid membrane-associated protein (ERMAP), also called Scianna blood group antigen, is a member of the butrophilin (BTN) and butrophilin-like (BTNL) family within the immunoglobulin superfamily. ERMAP consists of an extracellular domain (ECD) with one V-type Ig-like (IgV) fold, a transmembrane segment, and a cytoplasmic domain with a B30.2 motif (1).  Within the ECD, human ERMAP shares 33% and 31% amino acid sequence identity with mouse and rat ERMAP, respectively. Interestingly, human ERMAP contains a single IgV-like domain in the ECD, while mouse and rat ERMAP contain both an IgV-like and IgC-like domain. However, the absence of the IgC-like domain in human ERMAP does not seem to affect its functions (2, 3). ERMAP is an adhesion receptor molecule highly expressed in erythroid tissues, on the cell surface of resting and activated antigen-presenting cells (APCs) and in some tumor tissues (1, 4, 5). Fusion proteins (ERMAP-Ig) of both human and mouse ERMAP inhibit T cell functions in vitro and administration of the fusion protein ameliorates autoimmune diseases, including experimental autoimmune encephalomyelitis and type 1 diabetes, in mice (5). ERMAP acts as a novel inhibitory molecule for T cells and macrophages (5).