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Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

Catalog #: 287-TC
77 Citations 2 Reviews Datasheet / COA / SDS
Analyzed by SEC-MALS
Catalog # Availability Size / Price Qty
287-TC-500
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Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) Protein SEC-MALS.
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Citations (77)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) Summary

Product Specifications

Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.015-0.075 units/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin/EPO protein
Ala28-Arg193
Accession #
NP_000790.2
Predicted Molecular Mass
21 kDa
SDS-PAGE
37 kDa, reducing conditions

Product Datasheets

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287-TC

Product Datasheet
COA
SDS

287-TC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt at -20 to -70 °C as supplied.
  • 1 month at 2-8 °C under sterile conditions after reconstitution.
  • 3 months at -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant human EPO (Catalog # 287-TC) has a molecular weight (MW) of 28.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Bioactivity Graph showing dose-dependent bioactivity of Human Erythropoietin / EPO Protein View Larger

Recombinant Human Erythropoietin / EPO (287-TC) stimulates cell proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner. The ED50 for this effect is 0.015-0.075 units/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of the EPO protein is required for biological activities in vivo (4). The mature human EPO protein shares 75% - 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce erythropoietin production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

References
  1. Koury, M.J. (2005) Exp. Hematol. 33:1263.
  2. Jacobs, K. et al. (1985) Nature 313:806.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.
Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

Citations for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

77 Citations: Showing 1 - 10
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  1. Establishment of a transgene-free iPS cell line (SDCHi008-A) from a young patient bearing a KCNQ2 mutation and suffering from Epilepsy
    Authors: Zhang, H;Zhang, Q;Chen, X;Wang, M;Li, L;Yu, C;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Erythropoietin receptor signal is crucial for periodontal ligament stem cell-based tissue reconstruction in periodontal disease
    Authors: Zakaria, MF;Sonoda, S;Kato, H;Ma, L;Uehara, N;Kyumoto-Nakamura, Y;Sharifa, MM;Yu, L;Dai, L;Yamauchi-Tomoda, E;Aijima, R;Yamaza, H;Nishimura, F;Yamaza, T;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Establishment of a non-integrated iPSC line (SDQLCHi043-A) from a male infant with propionic acidemia carrying compound heterozygote mutations in PCCB gene
    Authors: Li, Z;Liu, C;Xin, H;Lv, Y;Gao, M;Ma, J;Liu, N;Gai, Z;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: BALF
    Applications: Bioassay
  4. Generation and characterization of PBMCs-derived human induced pluripotent stem cell (iPSC) line SDQLCHi050-A from a healthy donor
    Authors: Yang, X;Fan, Y;Li, Y;Zhang, H;Wang, B;Guan, J;Gao, J;Ma, X;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Establishment of human induced pluripotent stem cell line (SDQLCHi060-A) from a patient with AUTS2 syndrome carrying a heterozygous mutation in AUTS2 gene
    Authors: Gao, C;Liu, Y;Liu, N;Li, Z;Yang, X;Yang, Y;Xi, Y;Tian, J;Gai, Z;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Generation and characterization of a human induced pluripotent stem cell line (SDQLCHi052-A) from peripheral blood mononuclear cells derived from a healthy Chinese donor
    Authors: Wan, Z;Guan, J;Li, Y;Zhang, H;Wang, B;Yang, X;Jin, X;Ma, X;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Generation of a transgene-free iPS cell line (SDQLCHi053-A) from a young girl carrying a heterozygous mutation (c.427C > T) in SYNGAP1 gene
    Authors: Wang, Y;Yang, X;Zhang, H;Liu, N;Liu, Y;Gai, Z;Liu, Y;Lv, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Generation and characterization of PBMCs-derived human induced pluripotent stem cell (iPSC) line SDQLCHi051-A from an autism spectrum disorder patient with compound CHD8 gene mutations
    Authors: Fan, Y;Li, Y;Yang, X;Zhang, H;Wang, B;Guan, J;Gao, J;Ma, X;Liu, Y;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Generation of an induced pluripotent stem cell line SDPHi002-A from a patient with lung cancer
    Authors: D Ai, X Yang, X Li, N Liu, X Xie, Y Liu, W Song
    Stem Cell Research, 2023-04-15;69(0):103096.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Establishment of an induced pluripotent stem cell line SDQLCHi049-A from a healthy male neonate
    Authors: Y Xi, H Zhang, B Wang, W Song, Y Liu
    Stem Cell Research, 2023-04-15;69(0):103097.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Mapping of clonal lineages across developmental stages in human neural differentiation
    Authors: Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen
    Cell Stem Cell, 2023-03-17;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  12. Mapping of clonal lineages across developmental stages in human neural differentiation
    Authors: Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen
    Cell Stem Cell, 2023;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  13. Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftment
    Authors: X Wang, S Cooper, HE Broxmeyer, R Kapur
    Leukemia, 2022-12-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  14. Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture
    Authors: PY Su, W Lee, J Zernant, SH Tsang, T Nagasaki, B Corneo, R Allikmets
    Oncogene, 2022-11-14;65(0):102973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Generation of an induced pluripotent stem cell line (SDQLCHi044-A) from a patient with autosomal dominant mental retardation type 5 harboring heterozygous mutation in SYNGAP1 gene
    Authors: Y Wang, Y Lv, X Yang, Y Li, Z Li, Z Gao, Z Gai, Y Liu
    Stem Cell Research, 2022-09-17;64(0):102922.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells
    Authors: YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra
    Science Advances, 2022-08-24;8(34):eabn5522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Role of Erythropoietin Receptor Signaling in Macrophages or Choroidal Endothelial Cells in Choroidal Neovascularization
    Authors: A Ramshekar, CA Bretz, E Kunz, T Cung, BT Richards, GJ Stoddard, GS Hageman, B Chaqour, ME Hartnett
    Biomedicines, 2022-07-09;10(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells
    Authors: SA Luff, JP Creamer, S Valsoni, C Dege, R Scarfò, A Dacunto, S Cascione, LN Randolph, E Cavalca, I Merelli, SA Morris, A Ditadi, CM Sturgeon
    Nature Cell Biology, 2022-04-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker
    Stem Cell Research, 2021-12-23;59(0):102635.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
    Authors: AF de Souza, FF Bressan, NCG Pieri, RC Botigelli, T Revay, SK Haddad, DT Covas, ES Ramos, WA King, FV Meirelles
    Cells, 2021-11-10;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis
    Authors: ML Wegscheid, C Anastasaki, KA Hartigan, OM Cobb, JB Papke, JN Traber, SM Morris, DH Gutmann
    Cell Reports, 2021-07-06;36(1):109315.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  22. Generation of the iPSC line CUIMCi003-A derived from a patient with severe early onset obesity
    Authors: G Iannello, A Patel, D Sirabella, B Corneo, V Thaker
    Stem Cell Research, 2021-06-21;54(0):102432.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Generation of two human induced pluripotent stem cell (hiPSC) lines derived from unrelated Marfan Syndrome patients
    Authors: J Borsoi, M Morato-Mar, F de Araújo, L Assis Pere, LE Farinha-Ar, R Delgado Sa, AB Alvarez Pe, L Veiga Pere
    Stem Cell Research, 2021-05-25;54(0):102407.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene
    Authors: H Zhang, C Liu, Y Ma, L Lin, Y Lv, M Gao, Z Gai, Y Liu
    Stem Cell Research, 2021-04-09;53(0):102331.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene
    Authors: Z Li, X Zhang, X Li, Y Yang, H Xin, X Yang, N Liu, Z Gai, Y Liu
    Stem Cell Research, 2021-03-26;53(0):102313.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  26. Reprogramming of human peripheral blood mononuclear cell (PBMC) from a patient suffering from hearing loss into iPSC line (SDQLCHi035-A) maintaining compound heterozygous variations in GJB2 gene
    Authors: X Yang, N Liu, H Mu, Y Lv, H Zhang, Y Li, J Guan, Z Gai, Y Liu
    Stem Cell Research, 2021-01-21;51(0):102188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Establishment of a human induced pluripotent stem cell line (SDQLCHi037-A) from a patient with Alagille syndrome carrying heterozygous mutation in JAG1 gene
    Authors: B Wang, L Yang, Y Li, M Gao, H Zhang, X Yang, J Guan, Y Liu, Z Gai
    Stem Cell Research, 2021-01-08;51(0):102162.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. PBMC-derived integration-free iPSCs line SDQLCHi039-A from a patient with X-linked agammaglobulinemia carrying a novel 9-bp in-frame deletion in BTK gene
    Authors: N Liu, X Yang, S Wang, R Dong, Y Li, Y Lv, Y Liu, Z Gai
    Stem Cell Research, 2021-01-08;51(0):102165.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Generation and characterization of an induced pluripotent stem cell line SDQLCHi018-A from a congenital myasthenic syndrome patient carrying compound heterozygote mutations in RAPSN gene
    Authors: H Zhang, H Zhang, Y Ma, Y Lv, Z Gai, Y Liu
    Stem Cell Research, 2021-01-08;51(0):102160.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. An induced pluripotent stem cell line (SDQLCHi033-A) derived from a patient with maple syrup urine disease type Ib carrying a homozygous mutation in BCKDHB gene
    Authors: B Wang, C Liu, H Zhang, Z Gai, Y Liu
    Stem Cell Research, 2020-12-28;50(0):102146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Establishment of a non-integrate iPS cell line (SDQLCHi023-A) from a patient with Xq25 microduplication syndrome carrying a 1.3�Mb hemizygote duplication at chrXq25
    Authors: X Yang, C Duan, H Zhang, Y Li, J Guan, D Wang, Y Lv, Z Gai, Y Liu
    Stem Cell Research, 2020-12-28;51(0):102147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Modelling West Nile Virus and Usutu Virus Pathogenicity in Human Neural Stem Cells
    Authors: S Riccetti, A Sinigaglia, G Desole, N Nowotny, M Trevisan, L Barzon
    Viruses, 2020-08-12;12(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria
    Authors: H Yu, W Hu, X Song, D Descalzi-M, Z Yang, R Korngold, Y Zhao
    Int J Mol Sci, 2020-06-15;21(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Preclinical Evaluation of a Novel Lentiviral Vector Driving Lineage-Specific BCL11A Knockdown for Sickle Cell Gene Therapy
    Authors: C Brendel, O Negre, M Rothe, S Guda, G Parsons, C Harris, M McGuinness, D Abriss, A Tsytsykova, D Klatt, M Bentler, D Pellin, L Christians, A Schambach, J Manis, H Trebeden-N, M Bonner, E Esrick, G Veres, M Armant, DA Williams
    Mol Ther Methods Clin Dev, 2020-03-17;17(0):589-600.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Generation of patient-specific pluripotent induced stem cell line SDUBMSI002-A from a patient with X-linked mental retardation syndrome
    Authors: X Liu, X Yang, Y Li, X Wang, J Ma, W Jiang, Y Liu, W Sun, Y Gong
    Stem Cell Res, 2020-02-05;43(0):101724.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Generation of a human induced pluripotent stem cell line (SDUBMSi001-A) from a hereditary spastic paraplegia patient carrying kif1a c.773C>T missense mutation
    Authors: W Xiaojing, M Yanyan, D Ruonan, L Xiaolin, Z Haiyan, M Jian, L Yi, S Wenjie, L Qiji
    Stem Cell Res, 2020-02-04;43(0):101727.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  37. An integration-free iPSC line (SDQLCHi017-A) derived from a patient with nemaline myopathy-2 disease carrying compound heterozygote mutations in NEB gene
    Authors: Y Ma, H Zhang, X Li, X Yang, Y Li, J Guan, Y Lv, Z Gai, Y Liu
    Stem Cell Res, 2020-02-04;43(0):101729.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  38. Generation of a Human iPSC line (SDQLCHi021-A) from a patient with methylmalonic acidemia cblC type carrying compound heterozygous mutations in MMAHC gene
    Authors: J Guan, Z Li, H Zhang, X Yang, Y Ma, Y Li, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2020-01-16;43(0):101709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  39. In vitro activity of a G-quadruplex-stabilizing small molecule that synergizes with Navitoclax to induce cytotoxicity in acute myeloid leukemia cells
    Authors: JJ Montoya, MA Turnidge, DH Wai, AR Patel, DW Lee, V Gokhale, LH Hurley, RJ Arceci, C Wetmore, DO Azorsa
    BMC Cancer, 2019-12-27;19(1):1251.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49-50 exons in the DMD gene
    Authors: J Guan, X Liu, H Zhang, X Yang, Y Ma, Y Li, Z Gai, Y Liu
    Stem Cell Res, 2019-11-23;42(0):101666.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  41. Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene
    Authors: H Zhang, Y Ma, S Yu, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2019-10-21;41(0):101611.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  42. Generation of an iPSC line (SDQLCHi015-A) from peripheral blood mononuclear cells of a patient with mental retardation type 15 carrying c.1007_1011del, p.(Ile336fs) in CUL4B gene
    Authors: J Guan, X Liu, H Zhang, Y Lv, X Wang, X Yang, Y Ma, Q Liu, Y Liu, W Sun
    Stem Cell Res, 2019-10-18;41(0):101628.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  43. An integration-free iPSC line (SDQLCHi013-A) derived from a patient with maple syrup urine disease carrying compound heterozygote mutations in BCKDHA gene
    Authors: H Zhang, Y Ma, X Li, X Yang, Y Li, J Guan, R Dong, Z Gai, Y Liu
    Stem Cell Res, 2019-10-12;41(0):101585.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  44. An induced pluripotent stem cell line (SDQLCHi006-A) derived from a patient with maple syrup urine disease type Ib carrying compound heterozygous mutations of p.R168C and p.T322I in BCKDHB gene
    Authors: Y Li, H Zhang, B Yan, Y Ma, X Yang, J Guan, Y Lv, M Gao, J Ma, Z Gai, Y Liu
    Stem Cell Res, 2019-09-14;40(0):101579.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  45. An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease- 28 carrying compound heterozygote mutations in IL10RA gene
    Authors: Y Ma, H Zhang, S Zhang, R Dong, X Yang, Y Li, J Guan, Z Gai, Y Liu
    Stem Cell Res, 2019-09-13;41(0):101577.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  46. Establishment of a human induced pluripotent stem cell line (SDQLCHi004-A) from a patient with nemaline myopathy-4 disease carrying heterozygous mutation in TPM2 gene
    Authors: Y Ma, H Zhang, X Yang, Y Li, J Guan, Y Lv, H Li, Y Liu, Z Gai
    Stem Cell Res, 2019-09-10;40(0):101559.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Recombinant asialoerythropoetin protects HL-1 cardiomyocytes from injury via suppression of Mst1 activation
    Authors: FS Kittur, Y Lin, E Arthur, CY Hung, PA Li, DC Sane, J Xie
    Biochem Biophys Rep, 2019-01-09;17(0):157-168.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes
    Authors: L Cyganek, M Tiburcy, K Sekeres, K Gerstenber, H Bohnenberg, C Lenz, S Henze, M Stauske, G Salinas, WH Zimmermann, G Hasenfuss, K Guan
    JCI Insight, 2018-06-21;3(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Generation of Human Induced Pluripotent Stem Cells Using a Defined, Feeder-Free Reprogramming System.
    Authors: Park S, Mostoslavsky G
    Curr Protoc Stem Cell Biol, 2018-05-04;45(1):e48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Expression of mutant Asxl1 perturbs hematopoiesis and promotes susceptibility to leukemic transformation
    Authors: R Nagase, D Inoue, A Pastore, T Fujino, HA Hou, N Yamasaki, S Goyama, M Saika, A Kanai, Y Sera, S Horikawa, Y Ota, S Asada, Y Hayashi, KC Kawabata, R Takeda, HF Tien, H Honda, O Abdel-Waha, T Kitamura
    J. Exp. Med., 2018-04-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids
    Authors: MC Allenby, A Tahlawi, JCF Morais, K Li, N Panoskalts, A Mantalaris
    Stem Cells Int, 2018-04-02;2018(0):6230214.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. An inactivating mutation in the histone deacetylase SIRT6 causes human perinatal lethality
    Authors: CM Ferrer, M Alders, AV Postma, S Park, MA Klein, M Cetinbas, E Pajkrt, A Glas, S van Koning, VM Christoffe, MMAM Mannens, L Knegt, JP Etchegaray, RI Sadreyev, JM Denu, G Mostoslavs, MC van Maarle, R Mostoslavs
    Genes Dev., 2018-03-19;32(5):373-388.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. The effects of plasma gelsolin on human erythroblast maturation for erythrocyte production
    Authors: SY Han, EM Lee, HS Choi, BH Chun, EJ Baek
    Stem Cell Res, 2018-03-05;29(0):64-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Cytotoxicity Evaluation of Turmeric Extract Incorporated Oil-in-Water Nanoemulsion
    Authors: HJ Yoon, X Zhang, MG Kang, GJ Kim, SY Shin, SH Baek, BN Lee, SJ Hong, JT Kim, K Hong, H Bae
    Int J Mol Sci, 2018-01-17;19(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Erythropoietin promoted the proliferation of hepatocellular carcinoma through hypoxia induced translocation of its specific receptor
    Authors: S Miao, SM Wang, X Cheng, YF Li, QS Zhang, G Li, SQ He, XP Chen, P Wu
    Cancer Cell Int., 2017-12-11;17(0):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence
    Authors: N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra
    Nat Commun, 2017-05-25;8(0):15380.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Plant-Produced Asialo-Erythropoietin Restores Pancreatic Beta-Cell Function by Suppressing Mammalian Sterile-20-like Kinase (MST1) and Caspase-3 Activation
    Authors: E Arthur, FS Kittur, Y Lin, CY Hung, DC Sane, J Xie
    Front Pharmacol, 2017-04-19;8(0):208.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system
    Gene Ther., 2016-06-30;23(10):699-707.
    Species: Human
    Sample Types: Protein
    Applications: ELISA (Control)
  59. Glutathione peroxidase 4 prevents necroptosis in mouse erythroid precursors.
    Authors: Canli O, Alankus Y, Grootjans S, Vegi N, Hultner L, Hoppe P, Schroeder T, Vandenabeele P, Bornkamm G, Greten F
    Blood, 2015-10-13;127(1):139-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Production of Gene-Corrected Adult Beta Globin Protein in Human Erythrocytes Differentiated from Patient iPSCs After Genome Editing of the Sickle Point Mutation.
    Authors: Huang X, Wang Y, Yan W, Smith C, Ye Z, Wang J, Gao Y, Mendelsohn L, Cheng L
    Stem Cells, 2015-05-01;33(5):1470-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor.
    Authors: Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K
    Stem Cells, 2015-03-01;33(3):976-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  62. IDH2 mutation-induced histone and DNA hypermethylation is progressively reversed by small-molecule inhibition.
    Authors: Kernytsky A, Wang F, Hansen E, Schalm S, Straley K, Gliser C, Yang H, Travins J, Murray S, Dorsch M, Agresta S, Schenkein D, Biller S, Su S, Liu W, Yen K
    Blood, 2014-11-14;125(2):296-303.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014-07-02;124(9):1393-403.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Protein tyrosine phosphatase PTPN9 regulates erythroid cell development through STAT3 dephosphorylation in zebrafish.
    Authors: Bu Y, Su F, Wang X, Gao H, Lei L, Chang N, Wu Q, Hu K, Zhu X, Chang Z, Meng K, Xiong J
    J Cell Sci, 2014-04-11;127(0):2761-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. Calreticulin mutation-specific immunostaining in myeloproliferative neoplasms: pathogenetic insight and diagnostic value.
    Authors: Vannucchi, A M, Rotunno, G, Bartalucci, N, Raugei, G, Carrai, V, Balliu, M, Mannarelli, C, Pacilli, A, Calabresi, L, Fjerza, R, Pieri, L, Bosi, A, Manfredini, R, Guglielmelli, P
    Leukemia, 2014-03-12;28(9):1811-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Janus kinase 1/3 signaling pathways are key initiators of TH2 differentiation and lung allergic responses.
    Authors: Ashino S, Takeda K, Li H, Taylor V, Joetham A, Pine P, Gelfand E
    J Allergy Clin Immunol, 2013-12-22;133(4):1162-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. beta-Globin sleeping beauty transposon reduces red blood cell sickling in a patient-derived CD34(+)-based in vitro model.
    Authors: Sjeklocha L, Wong P, Belcher J, Vercellotti G, Steer C
    PLoS ONE, 2013-11-18;8(11):e80403.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  68. Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors.
    Authors: Huang, Xiaosong, Shah, Siddhart, Wang, Jing, Ye, Zhaohui, Dowey, Sarah N, Tsang, Kit Man, Mendelsohn, Laurel G, Kato, Gregory, Kickler, Thomas S, Cheng, Linzhao
    Mol Ther, 2013-09-03;22(2):451-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Discovery and characterization of LY2784544, a small-molecule tyrosine kinase inhibitor of JAK2V617F.
    Authors: Ma L, Clayton J, Walgren R, Zhao B, Evans R, Smith M, Heinz-Taheny K, Kreklau E, Bloem L, Pitou C, Shen W, Strelow J, Halstead C, Rempala M, Parthasarathy S, Gillig J, Heinz L, Pei H, Wang Y, Stancato L, Dowless M, Iversen P, Burkholder T
    Blood Cancer J, 2013-04-12;3(0):e109.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  70. Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity.
    Authors: McCloskey M, Wang H, Jiang Y, Smith G, Strange J, Hartnett M
    Invest Ophthalmol Vis Sci, 2013-03-21;54(3):2020-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  71. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
    Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
    Blood, 2011-11-23;119(7):1683-92.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  72. Characterization of BMS-911543, a functionally selective small-molecule inhibitor of JAK2.
    Authors: Purandare AV, McDevitt TM, Wan H
    Leukemia, 2011-10-21;26(2):280-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  73. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008-05-27;36(8):1035-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  74. Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo.
    Authors: Braun BS, Archard JA, Van Ziffle JA, Tuveson DA, Jacks TE, Shannon K
    Blood, 2006-05-23;108(6):2041-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  75. Virally mediated MafB transduction induces the monocyte commitment of human CD34+ hematopoietic stem/progenitor cells.
    Authors: Gemelli C, Montanari M, Tenedini E, Zanocco Marani T, Vignudelli T, Siena M, Zini R, Salati S, Tagliafico E, Manfredini R, Grande A, Ferrari S
    Cell Death Differ., 2006-02-03;13(10):1686-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  76. Growth hormone-induced stimulation of multilineage human hematopoiesis.
    Authors: Hanley MB, Napolitano LA, McCune JM
    Stem Cells, 2005-06-13;23(8):1170-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. Prospective isolation of human clonogenic common myeloid progenitors.
    Authors: Manz MG, 107325, Miyamoto T, Akashi K, Weissman IL
    Ultra-Sensitive and Semi-Quantitative Vertical Flow Assay for the Rapid Detection of Interleukin-6 in Inflammatory Diseases, 2002-08-22;99(18):11872-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Recombinant Human EPO is listed in units. How does this translate to mass?

    • One unit of Recombinant Human EPO (Ultra Pure; Catalog # 286-EP) or Recombinant Human EPO (Tissue Culture Grade; Catalog # 287-TC) has a mass of approximately 10 ng.

  2. How does 287-TC differ from other Recombinant Human Erythropoietin/EPO proteins?

    • 287-TC is considered Tissue Culture Grade. This product is tested for its ability to support the proliferation assay using TF‑1 human erythroleukemic cells. If additional testing specifications are required, the 11264-TC product is recommended. 287-TC is an excellent economical alternative intended for customers using large quantities of EPO for cultures.

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Reviews for Recombinant Human Erythropoietin/EPO (Tissue Culture Grade)

Average Rating: 5 (Based on 2 Reviews)

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Recombinant Human Erythropoietin (Tissue Culture Grade)
By Anonymous on 04/13/2018
Application: CellProlif
Reason for Rating: Using EPO as the primary cytokine to differentiate CD34 cells to mature erythrocytes/red blood cells in an 18 day culture

Using DRAQ-5 dye and GpA (erythroid cell marker) to determine levels of enucleation of differentiated cells in the Day 18 cultures. A large percentage of the cells have differentiated into mature reticulocytes/erythrocytes.

Cell Proliferation Recombinant Human Erythropoietin (Tissue Culture Grade) 287-TC
Recombinant Human Erythropoietin (Tissue Culture Grade)
By Leslie Priddy on 04/13/2018
Application: Cell migration/motility