Recombinant Human Erythropoietin R Fc Chimera Protein, CF

Catalog #: 963-ER
5 Citations Datasheet / COA / SDS

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963-ER-050

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Citations (5)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Erythropoietin R Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 1-4 ng/mL in the presence of 0.1 U/mL of recombinant human Epo.

Source

Mouse myeloma cell line, NS0-derived human Erythropoietin R protein

Human Epo R (Ala25-Pro250) Accession # P19235	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P19235

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51.4 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Product Datasheets

963-ER

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

963-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Erythropoietin R

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of EPO are mediated by the erythropoietin receptor (EPO R). The genes for human and mouse EPO R have been cloned and characterized. The full-length human EPO R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human EPO R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the EPO R gene, cDNA clones encoding a truncated form of the EPO R as well as a soluble form of EPO R has been found. The presence of a soluble form of the EPO R has also been detected in human sera. Recombinant soluble EPO R binds EPO with high affinity and is a potent EPO antagonist.

References

Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
Youssoufian, H. et al. (1993) Blood 9:2223.
Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
Baynes, R.D. et al. (1993) Blood 82:2088.

Long Name

Erythropoietin Receptor

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Alternate Names

EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

Related Research Areas

Citations for Recombinant Human Erythropoietin R Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Second-generation non-hematopoietic erythropoietin-derived peptide for neuroprotection
Authors: B Cho, SJ Yoo, SY Kim, CH Lee, YI Lee, SR Lee, C Moon
Redox Biology, 2021-12-21;49(0):102223.
Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance
The concentration of brain homogenates with the Amicon Ultra Centrifugal filters
Authors: J Yang, RK Sumbria
MethodsX, 2021-11-18;8(0):101584.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture
Targeted erythropoietin selectively stimulates red blood cell expansion in vivo.
Authors: DR Burrill, Burrill D, A Vernet, Vernet A, JJ Collins, PA Silver, JC Way, Way J
Proc Natl Acad Sci U S A, 2016-04-25;113(19):5245-50.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay
Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010-02-16;5(2):e9246.
Thrombopoietin contributes to enhanced platelet activation in cigarette smokers.
Authors: Lupia E, Bosco O, Goffi A
Atherosclerosis, 2009-11-20;210(1):314-9.
Species: Human
Sample Types: Plasma
Applications: Bioassay