Recombinant Human FCRL3/FcRH3 His-tag Protein, CF Summary
Product Specifications
Arg14-Arg569, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3126-FCB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Measured by its binding ability in a functional ELISA. When Recombinant Human FCRL3/FcRH3 His-tag Protein (Catalog # 3126-FCB) is immobilized at 2.00 μg/mL (100 μL/well), Normal Human IgG binds with an ED50 of 1.00‑6.00 μg/mL.
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2 μg/lane of Recombinant Human FCRL3/FcRH3 His-tag Protein (Catalog # 3126-FCB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-90 kDa.
Reconstitution Calculator
Background: FCRL3/FcRH3
FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2 - 4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).
- Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
- Miller, I. et al. (2002) Blood, 99:2662.
- Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
- Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
- Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
- Kochi, Y. et al. (2005) Nat. Genet. 37:478.
- Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.
Citations for Recombinant Human FCRL3/FcRH3 His-tag Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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MAIA, Fc receptor-like 3, supersedes JUNO as IZUMO1 receptor during human fertilization
Authors: J Vondrakova, M Frolikova, L Ded, J Cerny, P Postlerova, V Palenikova, O Simonik, Z Nahacka, K Basus, E Valaskova, R Machan, A Pacey, Z Holubcova, P Koubek, Z Ezrova, S Park, R Liu, R Partha, N Clark, J Neuzil, M Ikawa, K Erickson, KS Lam, H Moore, K Komrskova
Science Advances, 2022-09-07;8(36):eabn0047.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA
Authors: S Agarwal, Z Kraus, J Dement-Bro, O Alabi, K Starost, M Tolnay
Cell Rep, 2020-02-04;30(5):1292-1299.e3.
Species: Human
Sample Types: Protein, Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors.
Authors: Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M
J Immunol, 2013-04-24;190(11):5739-46.
Species: Human
Sample Types: Recombinant Proteins
Applications: Surface Plasmon Resonance (SPR
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