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Recombinant Human FGF-6 Protein

Catalog #: 238-F6
4 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
238-F6-025/CF

With Carrier

Catalog # Availability Size / Price Qty
238-F6-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF-6 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.1-0.3 ng/mL in the presence of 1 µg/mL of heparin.
Source
E. coli-derived human FGF-6 protein
Gly41-Ile208
Accession #
P10767.4
N-terminal Sequence
Analysis
Gly41 (minor) & Gly67 (major)
Predicted Molecular Mass
19 kDa (Gly41 - Ile208) & 16 kDa (Gly67 - Ile208)
SDS-PAGE
15-22 kDa, reducing conditions

Product Datasheets

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238-F6 (with carrier)

Product Datasheet
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238-F6/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

238-F6

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

238-F6/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-6

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25 - 28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5 - 7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7 - 9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

References
  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Marics, I. et al. (1989) Oncogene 4:335.
  4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
  6. Asada, M. et al. (1999) Growth Factors 16:293.
  7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
  8. de Lapeyriere, O. et al. (1993) Development 118:601.
  9. Li, J. et al. (1993) Cancer 72:2253.
  10. Floss, T. et al. (1997) Genes Dev. 11:2040.
  11. Grass, S. et al. (1996) Development 122:141.
    Long Name
    Fibroblast Growth Factor 6
    Entrez Gene IDs
    2251 (Human); 14177 (Mouse); 170700 (Rat)
    Alternate Names
    FGF6; FGF-6; fibroblast growth factor 6; HBGF-6; Heparin secretory-transforming protein 2; Heparin-binding growth factor 6; HST2; HST-2; HSTF2; HSTF-2

    Citations for Recombinant Human FGF-6 Protein

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    4 Citations: Showing 1 - 4
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    1. The FGF/FGFR system in the microglial neuroinflammation with Borrelia burgdorferi: likely intersectionality with other neurological conditions
      Authors: G Parthasara, MB Pattison, CC Midkiff
      Journal of Neuroinflammation, 2023-01-17;20(1):10.
      Species: Macaca mulatta (Rhesus Macaque)
      Sample Types: Whole Cells
      Applications: Bioassay
    2. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
      Authors: Tomlinson DC, Knowles MA
      Am. J. Pathol., 2010-10-01;177(5):2379-86.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    3. FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line.
      Authors: Israeli D, Benchaouir R, Ziaei S, Rameau P, Gruszczynski C, Peltekian E, Danos O, Garcia L
      J. Cell. Physiol., 2004-12-01;201(3):409-19.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    4. Increased expression of fibroblast growth factor 6 in human prostatic intraepithelial neoplasia and prostate cancer.
      Authors: Ropiquet F, Giri D, Kwabi-Addo B, Mansukhani A, Ittmann M
      Cancer Res., 2000-08-01;60(15):4245-50.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay

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