Recombinant Human FGFR5/FGFRL1 Protein, CF

Catalog #: 9805-FR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9805-FR-050

Recombinant Human FGFR5/FGFRL1 Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGFR5/FGFRL1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of MG-63 human bone fibroblasts. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human FGF R5/FGFRL1 protein
Ala25-Pro378, with a C-terminal 6-His tag

Accession #

Q8N441

N-terminal Sequence
Analysis

Ala25

Predicted Molecular Mass

40 kDa

SDS-PAGE

54-63 kDa, reducing conditions

Product Datasheets

9805-FR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9805-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Human FGF R5/FGFRL1 (Catalog # 9805-FR) supports MG-63 human bone Fibroblasts adhesion. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Reconstitution Calculator

Background: FGFR5/FGFRL1

Fibroblast growth factor receptor 5 (FGF R5), also known as FGRL1, is a 65 kDa transmembrane member of the FGF receptor family (1). Mature mouse FGF R5 consists of a 354 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 134 aa cytoplasmic domain (2). FGF R5 is distinct from other FGF receptor family members in that it does not contain a cytoplasmic tyrosine kinase domain (2, 3). Within the ECD, mouse FGF R5 shares 94% and 98% aa sequence identity with human and rat FGF R5, respectively. Alternate splicing results in an isoform that lacks the first Ig-like domain (2). FGF R5 is widely expressed, and expression of two species of mRNA in cartilage and pancreas may indicate the presence of both splice forms (2-6). Both the full length and truncated isoforms of FGF R5 bind FGF basic; the full length form additionally binds to heparin (2, 6). FGF R5 may function as a decoy receptor by binding FGF but not transducing its mitogenic signals (6). FGF R5 is found in most multicellular animals. It is known to bind to FGF ligands, with high affinity to FGF-3, -4, -8, -10, and -22 and weaker affinity to FGF-2, -5, -17, -18 and -23 (7). The extracellular domain of FGF R5 promotes cell adhesion when mediated by heparin sulfate glycosaminoglycans on the cell surface (8, 9). Comparing to the signaling receptors of FGFR family members (FGF R1-FGF R4), FGF R5 is more similar to the nectins for its adhesion properties and has no effect on cell growth and proliferation (9).

References

Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
Sleeman, M. et al. (2001) Gene 271:171.
Kim, I. et al. (2001) Biochim. Biophys. Acta 1518:152.
Antoine, M. et al. (2005) Growth Factors 23:87.
Wiedemann, M. et al. (2000) Genomics 69:275.
Trueb, B. et al. (2003) J. Biol. Chem. 278:33857.
Steinberg, F. et al (2010). J Biol Chem 285:2193.
Rieckmann, T. et al (2008) Exp Cell Res 314:1071.
Yang, X. et al (2016) International J Molec Med. 38:30.

Long Name

Fibroblast Growth Factor Receptor-like 1

Entrez Gene IDs

53834 (Human); 116701 (Mouse)

Alternate Names

FGF homologous factor receptor; FGF R5; FGF receptor-like protein 1; FGFR5; FGFR-5; FGFRL1; FGFR-like protein; FHFR; Fibroblast growth factor receptor 5; fibroblast growth factor receptor-like 1